Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

During development, cells in tissues must be patterned correctly in order to support tissue function and shape. The sensory bristles of the peripheral nervous system on the thorax of Drosophila melanogaster self-organizes from a unpatterned epithelial tissue to a regular spot pattern during pupal st...

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Published in:PloS one 2023-09, Vol.18 (9), p.e0291409-e0291409
Main Authors: Presser, Adam, Freund, Olivia, Hassapelis, Theodora, Hunter, Ginger
Format: Article
Language:English
Subjects:
Actin
Biology and Life Sciences
Bristles
Cells
Epithelial cells
Epithelium
Filopodia
Fruit flies
Genotype & phenotype
Insects
Ligands
Medicine and Health Sciences
Nervous system
Notch protein
Pattern formation
Patterning
Peripheral nervous system
Proteins
Research and Analysis Methods
Sensory bristles
Social Sciences
Stains & staining
Thorax
Tissues
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Freund, Olivia
Hassapelis, Theodora
Hunter, Ginger
description During development, cells in tissues must be patterned correctly in order to support tissue function and shape. The sensory bristles of the peripheral nervous system on the thorax of Drosophila melanogaster self-organizes from a unpatterned epithelial tissue to a regular spot pattern during pupal stages. Wild type patterning requires Notch-mediated lateral inhibition. Scabrous is a protein that can bind to and modify Notch receptor activity. Scabrous can be secreted, but it is also known to be localized to basal signaling filopodia, or cytonemes, that play a role in long-range Notch signaling. Here we show that Scabrous is primarily distributed basally, within the range of signaling filopodia extension. We show that filamentous actin dynamics are required for the distribution of Scabrous protein during sensory bristle patterning stages. We show that the Notch response of epithelial cells is sensitive to the level of Scabrous protein being expressed by the sensory bristle precursor cell. Our findings at the cell-level suggest a model for how epithelial cells engaged in lateral inhibition at a distance are sensitive local levels of Scabrous protein.
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subjects Actin
Biology and Life Sciences
Bristles
Cells
Epithelial cells
Epithelium
Filopodia
Fruit flies
Genotype & phenotype
Insects
Ligands
Medicine and Health Sciences
Nervous system
Notch protein
Pattern formation
Patterning
Peripheral nervous system
Proteins
Research and Analysis Methods
Sensory bristles
Social Sciences
Stains & staining
Thorax
Tissues
title Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila
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