A chemical signal in human female tears lowers aggression in males

• Published in: PLoS biology 2023-12, Vol.21 (12), p.e3002442-e3002442
• Main Authors: Agron, Shani, de March, Claire A, Weissgross, Reut, Mishor, Eva, Gorodisky, Lior, Weiss, Tali, Furman-Haran, Edna, Matsunami, Hiroaki, Sobel, Noam
• Format: Article
• Language: English
• Subjects: Aggression; Aggression - physiology; Aggressiveness; Aggressiveness (Psychology); Analysis; Behavior; Biology and Life Sciences; Blocking; Brain; Brain - physiology; Brain research; Chemoreception; Computer and Information Sciences; Exocrine glands; Experiments; Female; Females; Human acts; Human behavior; Humans; Life Sciences; Male; Males; Medicine and Health Sciences; Neural networks; Neuroimaging; Odorant receptors; Odorants; Odors; Olfaction; Peptides; Receptors; Research and Analysis Methods; Rodents; Short Reports; Smell - physiology; Social Sciences; Structure-function relationships; Substrates; Tearing; Tears - chemistry; Testosterone; Testosterone - pharmacology
• ISSN: 1545-7885; 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.3002442

Rodent tears contain social chemosignals with diverse effects, including blocking male aggression. Human tears also contain a chemosignal that lowers male testosterone, but its behavioral significance was unclear. Because reduced testosterone is associated with reduced aggression, we tested the hypothesis that human tears act like rodent tears to block male aggression. Using a standard behavioral paradigm, we found that sniffing emotional tears with no odor percept reduced human male aggression by 43.7%. To probe the peripheral brain substrates of this effect, we applied tears to 62 human olfactory receptors in vitro. We identified 4 receptors that responded in a dose-dependent manner to this stimulus. Finally, to probe the central brain substrates of this effect, we repeated the experiment concurrent with functional brain imaging. We found that sniffing tears increased functional connectivity between the neural substrates of olfaction and aggression, reducing overall levels of neural activity in the latter. Taken together, our results imply that like in rodents, a human tear-bound chemosignal lowers male aggression, a mechanism that likely relies on the structural and functional overlap in the brain substrates of olfaction and aggression. We suggest that tears are a mammalian-wide mechanism that provides a chemical blanket protecting against aggression.