Near-infrared spectroscopy and machine learning algorithms for rapid and non-invasive detection of Trichuris

• Published in: PLoS neglected tropical diseases 2023-11, Vol.17 (11), p.e0011695-e0011695
• Main Authors: Kariyawasam, Tharanga N, Ciocchetta, Silvia, Visendi, Paul, Soares Magalhães, Ricardo J, Smith, Maxine E, Giacomin, Paul R, Sikulu-Lord, Maggy T
• Format: Article
• Language: English
• Subjects: Algorithms; Analysis; Animal experimentation; Animal models; Artificial neural networks; Blood; Care and treatment; Data analysis; Data mining; Diagnosis; Eggs; Ethics; Experiments; Fiber optics; Food contamination & poisoning; Health aspects; Helminthiasis; Human populations; Infections; Infrared spectra; Infrared spectroscopy; Large intestine; Learning algorithms; Machine learning; Malaria; Microscopy; Near infrared radiation; Neural networks; Parasites; Performance assessment; Serum; Skin; Software; Spectrum analysis; Surveillance; Tropical diseases
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0011695

Background Trichuris trichiura (whipworm) is one of the most prevalent soil transmitted helminths (STH) affecting 604-795 million people worldwide. Diagnostic tools that are affordable and rapid are required for detecting STH. Here, we assessed the performance of the near-infrared spectroscopy (NIRS) technique coupled with machine learning algorithms to detect Trichuris muris in faecal, blood, serum samples and non-invasively through the skin of mice. Methodology We orally infected 10 mice with 30 T. muris eggs (low dose group), 10 mice with 200 eggs (high dose group) and 10 mice were used as the control group. Using the NIRS technique, we scanned faecal, serum, whole blood samples and mice non-invasively through their skin over a period of 6 weeks post infection. Using artificial neural networks (ANN) and spectra of faecal, serum, blood and non-invasive scans from one experiment, we developed 4 algorithms to differentiate infected from uninfected mice. These models were validated on mice from a second independent experiment. Principal findings NIRS and ANN differentiated mice into the three groups as early as 2 weeks post infection regardless of the sample used. These results correlated with those from concomitant serological and parasitological investigations. Significance To our knowledge, this is the first study to demonstrate the potential of NIRS as a diagnostic tool for human STH infections. The technique could be further developed for large scale surveillance of soil transmitted helminths in human populations.