A Prospective cohort study of zoonotic cutaneous leishmaniasis in tunisia: Clinical and Immunological features and immune correlates of protection

• Published in: PLoS neglected tropical diseases 2023-12, Vol.17 (12), p.e0011784-e0011784
• Main Authors: Naouar, Ikbel, Kammoun Rebai, Wafa, Ben Salah, Afif, Bouguerra, Hind, Toumi, Amine, Hamida, Nabil Belhadj, Louzir, Hechmi, Meddeb-Garnaoui, Amel
• Format: Article
• Language: English
• Subjects: Biological response modifiers; Biology and Life Sciences; Blood; Care and treatment; Cohorts; Correlation; Cutaneous leishmaniasis; Diagnosis; Disease transmission; Foci; Granzyme B; Health aspects; Heterogeneity; Immunology; Infection; Infections; Interferon gamma; Interleukin 10; Interleukins; Leishmaniasis; Leishmanin; Leukocytes (mononuclear); Lymphocytes; Medical research; Medicine and Health Sciences; Medicine, Experimental; Parasites; Parasitic diseases; Peripheral blood mononuclear cells; Population studies; Protozoa; Public health; Seasons; Skin; Skin tests; Tumor necrosis factor-TNF; Vector-borne diseases; Zoonoses; γ-Interferon
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0011784

This study aimed to define immunological markers of exposure to L. major parasites and identify correlates of protection against infection. We analyzed a cohort of 790 individuals at risk of developing ZCL living in endemic areas with varying L. major infection prevalence. One area had a high infection prevalence indicated by high proportions of leishmanin skin test (LST) positive subjects, while the other areas were recent foci with lower infection prevalence. Blood samples were collected before the transmission season to measure Interferon gamma (IFN-γ), Interleukin 10 (IL-10), and Granzyme B (GrB) levels in response to parasite stimulation in peripheral blood mononuclear cells. A one-year follow-up period involved active detection of new ZCL cases to estimate disease incidence after a transmission season and identify immune correlates of protection. The study population showed heterogeneity in parasite contact, evident from specific scars and/or positive LST results, significantly higher in the old focus compared to recent foci. IFN-γ and GrB were markers of parasite exposure and reliable indicators of immunity to L. major. Positive correlations were observed between IFN-γ/IL-10 and GrB/IL-10 ratios and LST results. Unexpectedly, only 29 new ZCL cases (4%) appeared after a transmission season, with 27 cases reported in recent foci and 2 in the oldest focus. Our findings indicate that individuals in L. major endemic areas are likely to develop ZCL regardless of their LST status. We showed that high pre-transmission season levels of IFN-γ and GrB produced by PBMC, along with a high IFN-γ/IL-10 ratio, were associated with protection. This study on a large cohort at risk of ZCL confirmed IFN-γ and GrB as protective factors against the disease. A high IFN-γ/IL-10 ratio, but not GrB/IL-10 ratio was associated with resistance. These results are valuable for developing and evaluating of a vaccine against human leishmaniasis.