Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

• Published in: PloS one 2023-11, Vol.18 (11), p.e0286349-e0286349
• Main Authors: Ismail, Hammad, Khalid, Dania, Waseem, Durdana, Ijaz, Muhammad Umar, Dilshad, Erum, Haq, Ihsan-ul, Bhatti, Muhammad Zeeshan, Anwaar, Sadaf, Ahmed, Madiha, Saleem, Samreen
• Format: Article
• Language: English
• Subjects: Acetates; Acetic acid; Acetylcholinesterase; Aluminum; Aluminum chloride; Aluminum compounds; Alzheimer's disease; Analysis; Berberine; Berberis lycium; Bioassays; Biochemical assays; Biology and Life Sciences; Brain; Care and treatment; Chemical tests and reagents; Chlorides; Chromatography; Column chromatography; Developing countries; Diagnosis; Dopamine; Enzymes; Esters; Ethyl acetate; Flavonoids; Fractionation; Herbal medicine; High performance liquid chromatography; In vivo methods and tests; Indigenous plants; LDCs; Liquid chromatography; Medical care, Cost of; Medicinal plants; Medicine and Health Sciences; Methanol; Methods; Molecular docking; Neurodegenerative diseases; Neuroprotection; NMR; Nuclear magnetic resonance; Older people; Physical Sciences; Phytochemicals; Potassium; Research and Analysis Methods; Rivastigmine; Secretase; Serotonin; Tropical diseases
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0286349

Berberis lycium is an indigenous plant of Pakistan that is known for its medicinal properties. In the current study, we investigated the anti-Alzheimer's effect of berberine isolated from Berberis lycium. Root extract of B. lycium was subjected to acetylcholinesterase inhibition assay and column chromatography for bioassays guided isolation of a compound. The neuroprotective and memory improving effects of isolated compound were evaluated by aluminium chloride induced Alzheimer's disease rat model, elevated plus maze (EPM) and Morris water maze (MWM) tests., Levels of dopamine and serotonin in rats brains were determined using HPLC. Moreover, western blot and docking were performed to determine interaction between berberine and [beta]-secretase. During fractionation, ethyl acetate and methanol (3:7) fraction was collected from solvent mixture of ethyl acetate and methanol. This fraction showed the highest anti-acetylcholinesterase activity and was alkaloid positive. The results of TLC and HPLC analysis indicated the presence of the isolated compound as berberine. Additionally, the confirmation of isolated compound as berberine was carried out using FTIR and NMR analysis. In vivo EPM and MWM tests showed improved memory patterns after berberine treatment in Alzheimer's disease model. The levels of dopamine, serotonin and activity of antioxidant enzymes were significantly (p