Validation of dual energy X-ray absorptiometry for longitudinal quantification of tumor burden in a murine model of pancreatic ductal adenocarcinoma

Noninvasive imaging is central to preclinical, in vivo models of pancreatic ductal adenocarcinoma (PDAC). While bioluminescent imaging (BLI) is a gold standard, its signal is dependent on the metabolic activity of tumor cells. In contrast, dual energy X-ray absorptiometry (DEXA) is a direct measure...

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Bibliographic Details
Published in:PloS one 2024-01, Vol.19 (1), p.e0292196-e0292196
Main Authors: Sechrist, Zachary R, Lee, Grace, Schwarz, Edward M, Cole, Calvin L
Format: Article
Language:English
Subjects:
Abdomen
Absorptiometry, Photon - methods
Adenocarcinoma
Analysis
Animal models
Animals
Biology and Life Sciences
Body composition
Bone densitometry
Carcinoma, Pancreatic Ductal - diagnostic imaging
Care and treatment
Diagnosis
Disease Models, Animal
Dual energy X-ray absorptiometry
Failure to thrive
Inoculation
Kidney cancer
Laboratory animals
Luciferase
Medical research
Medicine and Health Sciences
Mice
Musculoskeletal system
Pancreas
Pancreatic cancer
Pancreatic Neoplasms - diagnostic imaging
Research and Analysis Methods
Respiration
Tumor Burden
Tumor cells
Tumors
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Summary:Noninvasive imaging is central to preclinical, in vivo models of pancreatic ductal adenocarcinoma (PDAC). While bioluminescent imaging (BLI) is a gold standard, its signal is dependent on the metabolic activity of tumor cells. In contrast, dual energy X-ray absorptiometry (DEXA) is a direct measure of body composition. Thus, we aimed to assess its potential for longitudinal quantification of tumor burden versus BLI. We utilized the KCKO murine model of PDAC and subjected tumor-bearing (n = 20) and non-tumor control (NTC) (n = 10) animals to weekly BLI and DEXA measurements for up to 10 weeks. While BLI detected tumors at 1-week, it failed to detect tumor growth, displayed a decreasing trend overtime (slope = -9.0x108; p = 0.0028), and terminal signal did not correlate with ex vivo tumor mass (r = 0.01853; p = 0.6286). In contrast, DEXA did not detect elevated changes in abdominal cavity lean mass until week 2 post inoculation and tumors were not visible until week 3, but successfully quantified a tumor growth trend (slope = 0.7322; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0292196