Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model

• Published in: PloS one 2024-01, Vol.19 (1), p.e0292050-e0292050
• Main Authors: Roh, Yoon Jin, Choi, Yong Hee, Shin, Sun Hye, Lee, Mi-Kyung, Won, Yu Jin, Lee, Jun Ho, Cho, Byong Seung, Park, Kui Young, Seo, Seong Jun
• Format: Article
• Language: English
• Subjects: Adipose tissue; Adipose Tissue - metabolism; Adipose tissues; Advertising executives; Air pollution; Analysis; Antibodies; Atopic dermatitis; Body fat; Care and treatment; Cell culture; Composition; Cytokines; Cytokines - metabolism; Damage; Dermatitis; Dermatitis, Atopic - drug therapy; Dermatitis, Atopic - therapy; Diagnosis; Ethylenediaminetetraacetic acid; Exosomes; Exosomes - metabolism; Filaggrin; Gene expression; Health aspects; Humans; Immunofluorescence; Immunomodulation; Inflammation; Inflammation - metabolism; Inflammatory response; Interleukin 1; Interleukins; Keratinocytes; Mast cells; Measurement; Mesenchymal stem cells; Outdoor air quality; Particles; Particulate emissions; Particulate matter; Particulate Matter - metabolism; Particulate Matter - toxicity; Pharmacology; Polyinosinic:polycytidylic acid; Polymerase chain reaction; Proteins; Real time; RNA; Skin; Skin - metabolism; Stem cells; Western blotting; Wound healing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0292050

Recently, particulate matter (PM) has been shown to exacerbate atopic dermatitis (AD) by inducing an inflammatory response. Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD.