Isolation and screening of fungi for enhanced agarwood formation in Aquilaria sinensis trees

Agarwood is a resinous heartwood of Aquilaria sinensis that is formed in response to mechanical wounding. In the present study pre-treatment of Aquilaria sinensis was carried out, and then the dominant fungi were isolated and purified from the surface and electroshock holes of trees. The isolated Tr...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2024-06, Vol.19 (6), p.e0304946
Main Authors: Liu, Chuang, Zhou, Guoying, Liu, Junang
Format: Article
Language:English
Subjects:
Acetone
Aquilaria sinensis
Biology and Life Sciences
Chemical composition
Chromatography
Constituents
Electroconvulsive therapy
Fungal infections
Fungi
Gas chromatography
Gas Chromatography-Mass Spectrometry
Genetic testing
Incense
Mass spectrometry
Mass spectroscopy
Neurospora
Physical Sciences
Resins
Spectroscopy
Thymelaeaceae - chemistry
Thymelaeaceae - microbiology
Trees
Trees - microbiology
Trichoderma
Trichoderma - isolation & purification
Trichoderma - metabolism
Wood - chemistry
Wood - microbiology
Wounding
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Agarwood is a resinous heartwood of Aquilaria sinensis that is formed in response to mechanical wounding. In the present study pre-treatment of Aquilaria sinensis was carried out, and then the dominant fungi were isolated and purified from the surface and electroshock holes of trees. The isolated Trichoderma sp. and Neurospora sp. were then screened for resistance against benzyl acetone and then inoculated into healthy Aquilaria sinensis trees. After six months, the agarwood was collected for analysis. The chemical composition of incense was analyzed using gas chromatography-mass spectroscopy, and 82 chemical constituents were identified. Agarwood products formed by using Trichoderma sp. and Neurospora sp. consisted of 50.22% and 48.71% ether extracts, respectively, which surpassed the 10% threshold specified by the Chinese Pharmacopoeia. Similarly, relative aromatic contents in the two agarwood products were 30.1% and 32.86%, while proportions of sesquiterpene constituents were 10.21% and 11.19%, respectively. These two agarwood-specific chemical constituents accounted for a large proportion of the total chemical composition, which showed that the generated agarwood was of good quality. The results of the study revealed that both Trichoderma sp. and Neurospora sp. were able to effectively induce agarwood production in Aquilaria sinensis trees in 6 months. This study expands the library of fungi that promote the production of agarwood from Aquilaria sinensis trees.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0304946