Pyramiding of bacterial blight resistance genes into promising restorer BRRI31R line through marker-assisted backcross breeding and evaluation of agro-morphological and physiochemical characteristics of developed resistant restorer lines

BRRI31R is one of the Bangladesh's most promising restorer lines due to its abundant pollen producing capacity, strong restoring ability, good combining ability, high outcrossing rate and genetically diverse from cytoplasmic male sterile (CMS) line. But the drawback of this line is that it is h...

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Bibliographic Details
Published in:PloS one 2024-06, Vol.19 (6), p.e0301342
Main Authors: Akter, Anowara, Hassan, Lutful, Nihad, Sheikh Arafat Islam, Hasan, Md Jamil, Robin, Arif Hasan Khan, Khatun, Mahmuda, Tabassum, Anika, Latif, Mohammad Abdul
Format: Article
Language:English
Subjects:
Agricultural production
Amylose
Analysis
Antibiotics
Bacterial genetics
Biology and Life Sciences
Blast resistance
Blight
Combining ability
Crop diseases
Crop production
Crosses, Genetic
Cytoplasmic male sterility
Disease resistance
Disease Resistance - genetics
Fertility
Genes
Genes, Plant
Genetic Markers
Genotyping
Males
Medicine and Health Sciences
Morphology
Oryza - genetics
Oryza - microbiology
Pathogenicity
Pathogens
People and Places
Physical characteristics
Physiochemistry
Plant Breeding
Plant Diseases - genetics
Plant Diseases - microbiology
Pollen
Races
Research and Analysis Methods
Rice
Seeds
Single-nucleotide polymorphism
Xanthomonas - genetics
Xanthomonas - pathogenicity
Xanthomonas oryzae
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Summary:BRRI31R is one of the Bangladesh's most promising restorer lines due to its abundant pollen producing capacity, strong restoring ability, good combining ability, high outcrossing rate and genetically diverse from cytoplasmic male sterile (CMS) line. But the drawback of this line is that it is highly susceptible to bacterial blight (BB) disease of rice caused by Xanthomonas oryzae pv. oryzae. The present study highlighted the pyramiding of effective BB resistance genes (xa5, xa13 and Xa21) into the background of BRRI31R, through marker-assisted backcrossing (MABC). Backcross progenies were confirmed and advanced based on the foreground selection of target genes. Pyramided lines were used for pathogenicity test against five Bangladeshi Xanthomonas oryzae (BXo) races (BXo93, BXo220, BXo822, BXo826, BXo887) and confirmed the dominant fertility restore genes, Rf3 and Rf4 and further validated against SNP markers for more confirmation of target resistance genes. All pyramided restorer lines consisted of Xa4 (in built), xa5, xa13, Xa21, and Chalk5 with two fertility restorer genes, Rf3, Rf4. and these restorer lines showed intermediate amylose content (
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0301342