The impact of microplastics polystyrene on the microscopic structure of mouse intestine, tight junction genes and gut microbiota

Microplastics, which are tiny plastic particles less than 5 mm in diameter, are widely present in the environment, have become a serious threat to aquatic life and human health, potentially causing ecosystem disorders and health problems. The present study aimed to investigate the effects of micropl...

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Published in:PloS one 2024-06, Vol.19 (6), p.e0304686-e0304686
Main Authors: Su, Qi-Ling, Wu, Jiang, Tan, Shao-Wen, Guo, Xiao-Yun, Zou, Ding-Zhe, Kang, Kai
Format: Article
Language:English
Subjects:
Animals
Aquatic organisms
Biological diversity
Biology and Life Sciences
Cell junctions
Claudin-1 - genetics
Claudin-1 - metabolism
Claudins - genetics
Claudins - metabolism
Digestive system
Duodenum
Dysbacteriosis
Ecology and Environmental Sciences
Ecosystems
Environmental aspects
Excretory system
Exposure
Female
Females
Gastrointestinal Microbiome - drug effects
Gastrointestinal system
Gastrointestinal tract
Gene expression
Genes
Genetic aspects
Health aspects
Health problems
Height
Intestinal microflora
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestinal Mucosa - microbiology
Intestine
Jejunum
Junctional complexes (Epithelium)
Male
Males
Medicine and Health Sciences
Mice
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Microplastics
Microplastics - toxicity
Nucleotides
Occludin - genetics
Occludin - metabolism
Permeability
Physiological aspects
Physiology
Plastic pollution
Political aspects
Polystyrene
Polystyrene resins
Polystyrenes - toxicity
Proteins
Relative abundance
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Small intestine
Structural integrity
Tight Junction Proteins - genetics
Tight Junction Proteins - metabolism
Tight junctions
Tight Junctions - drug effects
Tight Junctions - metabolism
Villus
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Wu, Jiang
Tan, Shao-Wen
Guo, Xiao-Yun
Zou, Ding-Zhe
Kang, Kai
description Microplastics, which are tiny plastic particles less than 5 mm in diameter, are widely present in the environment, have become a serious threat to aquatic life and human health, potentially causing ecosystem disorders and health problems. The present study aimed to investigate the effects of microplastics, specifically microplastics-polystyrene (MPs-PS), on the structural integrity, gene expression related to tight junctions, and gut microbiota in mice. A total of 24 Kunming mice aged 30 days were randomly assigned into four groups: control male (CM), control female (CF), PS-exposed male (PSM), and PS-exposed female (PSF)(n = 6). There were significant differences in villus height, width, intestinal surface area, and villus height to crypt depth ratio (V/C) between the PS group and the control group(C) (p
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The present study aimed to investigate the effects of microplastics, specifically microplastics-polystyrene (MPs-PS), on the structural integrity, gene expression related to tight junctions, and gut microbiota in mice. A total of 24 Kunming mice aged 30 days were randomly assigned into four groups: control male (CM), control female (CF), PS-exposed male (PSM), and PS-exposed female (PSF)(n = 6). There were significant differences in villus height, width, intestinal surface area, and villus height to crypt depth ratio (V/C) between the PS group and the control group(C) (p <0.05). Gene expression analysis demonstrated the downregulation of Claudin-1, Claudin-2, Claudin-15, and Occludin, in both duodenum and jejunum of the PS group (p < 0.05). Analysis of microbial species using 16S rRNA sequencing indicated decreased diversity in the PSF group, as well as reduced diversity in the PSM group at various taxonomic levels. Beta diversity analysis showed a significant difference in gut microbiota distribution between the PS-exposed and C groups (R2 = 0.113, p<0.01), with this difference being more pronounced among females exposed to MPs-PS. KEGG analysis revealed enrichment of differential microbiota mainly involved in seven signaling pathways, such as nucleotide metabolism(p<0.05). The relative abundance ratio of transcriptional pathways was significantly increased for the PSF group (p<0.01), while excretory system pathways were for PSM group(p<0.05). 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Qi-Ling</au><au>Wu, Jiang</au><au>Tan, Shao-Wen</au><au>Guo, Xiao-Yun</au><au>Zou, Ding-Zhe</au><au>Kang, Kai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impact of microplastics polystyrene on the microscopic structure of mouse intestine, tight junction genes and gut microbiota</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-06-05</date><risdate>2024</risdate><volume>19</volume><issue>6</issue><spage>e0304686</spage><epage>e0304686</epage><pages>e0304686-e0304686</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Microplastics, which are tiny plastic particles less than 5 mm in diameter, are widely present in the environment, have become a serious threat to aquatic life and human health, potentially causing ecosystem disorders and health problems. The present study aimed to investigate the effects of microplastics, specifically microplastics-polystyrene (MPs-PS), on the structural integrity, gene expression related to tight junctions, and gut microbiota in mice. A total of 24 Kunming mice aged 30 days were randomly assigned into four groups: control male (CM), control female (CF), PS-exposed male (PSM), and PS-exposed female (PSF)(n = 6). There were significant differences in villus height, width, intestinal surface area, and villus height to crypt depth ratio (V/C) between the PS group and the control group(C) (p <0.05). Gene expression analysis demonstrated the downregulation of Claudin-1, Claudin-2, Claudin-15, and Occludin, in both duodenum and jejunum of the PS group (p < 0.05). Analysis of microbial species using 16S rRNA sequencing indicated decreased diversity in the PSF group, as well as reduced diversity in the PSM group at various taxonomic levels. Beta diversity analysis showed a significant difference in gut microbiota distribution between the PS-exposed and C groups (R2 = 0.113, p<0.01), with this difference being more pronounced among females exposed to MPs-PS. KEGG analysis revealed enrichment of differential microbiota mainly involved in seven signaling pathways, such as nucleotide metabolism(p<0.05). The relative abundance ratio of transcriptional pathways was significantly increased for the PSF group (p<0.01), while excretory system pathways were for PSM group(p<0.05). Overall findings suggest that MPs-PS exhibit a notable sex-dependent impact on mouse gut microbiota, with a stronger effect observed among females; reduced expression of tight junction genes may be associated with dysbiosis, particularly elevated levels of Prevotellaceae.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38837998</pmid><doi>10.1371/journal.pone.0304686</doi><tpages>e0304686</tpages><orcidid>https://orcid.org/0000-0002-0771-1791</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2024-06, Vol.19 (6), p.e0304686-e0304686
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_3069270079
source PubMed Central Free; Publicly Available Content (ProQuest)
subjects Animals
Aquatic organisms
Biological diversity
Biology and Life Sciences
Cell junctions
Claudin-1 - genetics
Claudin-1 - metabolism
Claudins - genetics
Claudins - metabolism
Digestive system
Duodenum
Dysbacteriosis
Ecology and Environmental Sciences
Ecosystems
Environmental aspects
Excretory system
Exposure
Female
Females
Gastrointestinal Microbiome - drug effects
Gastrointestinal system
Gastrointestinal tract
Gene expression
Genes
Genetic aspects
Health aspects
Health problems
Height
Intestinal microflora
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestinal Mucosa - microbiology
Intestine
Jejunum
Junctional complexes (Epithelium)
Male
Males
Medicine and Health Sciences
Mice
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Microplastics
Microplastics - toxicity
Nucleotides
Occludin - genetics
Occludin - metabolism
Permeability
Physiological aspects
Physiology
Plastic pollution
Political aspects
Polystyrene
Polystyrene resins
Polystyrenes - toxicity
Proteins
Relative abundance
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Small intestine
Structural integrity
Tight Junction Proteins - genetics
Tight Junction Proteins - metabolism
Tight junctions
Tight Junctions - drug effects
Tight Junctions - metabolism
Villus
title The impact of microplastics polystyrene on the microscopic structure of mouse intestine, tight junction genes and gut microbiota
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