Rv0100: An essential acyl carrier protein from M. tuberculosis important in dormancy

We have identified an acyl-carrier protein, Rv0100, that is up-regulated in a dormancy model. This protein plays a critical role in the fatty acid biosynthesis pathway, which is important for energy storage and cell wall synthesis in Mycobacterium tuberculosis (MTB). Knocking out the Rv0100 gene res...

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Bibliographic Details
Published in:PloS one 2024-06, Vol.19 (6), p.e0304876
Main Authors: Gutka, Hiten J, Bondoc, Jasper Marc G, Patwell, Ryan, Khan, Shahebraj, Grzelak, Edyta M, Goswami, Rajendra, Voskuil, Martin I, Movahedzadeh, Farahnaz
Format: Article
Language:English
Subjects:
Acyl carrier protein
Acyl Carrier Protein - genetics
Acyl Carrier Protein - metabolism
Animals
Antibacterial agents
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biology and Life Sciences
Biosynthesis
Cell walls
Cloning
Dormancy
Drug resistance
Energy storage
Enzymes
Fatty acids
Gene Expression Regulation, Bacterial
Genes
Genomes
Health aspects
Hypoxia
Infections
Latent infection
Leprosy
Lipids
Lipids - chemistry
Macrophages
Macrophages - metabolism
Macrophages - microbiology
Medicine and Health Sciences
Methylene blue
Mice
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - metabolism
Mycobacterium tuberculosis - pathogenicity
Pathogens
Peptides
Physiological aspects
Plasmids
Polymerase chain reaction
Proteins
Research and Analysis Methods
Tuberculosis
Tuberculosis - microbiology
Virulence
Virulence (Microbiology)
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Summary:We have identified an acyl-carrier protein, Rv0100, that is up-regulated in a dormancy model. This protein plays a critical role in the fatty acid biosynthesis pathway, which is important for energy storage and cell wall synthesis in Mycobacterium tuberculosis (MTB). Knocking out the Rv0100 gene resulted in a significant reduction of growth compared to wild-type MTB in the Wayne model of non-replicating persistence. We have also shown that Rv0100 is essential for the growth and survival of this pathogen during infection in mice and a macrophage model. Furthermore, knocking out Rv0100 disrupted the synthesis of phthiocerol dimycocerosates, the virulence-enhancing lipids produced by MTB and Mycobacterium bovis. We hypothesize that this essential gene contributes to MTB virulence in the state of latent infection. Therefore, inhibitors targeting this gene could prove to be potent antibacterial agents against this pathogen.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0304876