Mesencephalic trigeminal nucleus neurons with collaterals to both eyelid and masseter muscles shown by fluorescent double-labeling, revealing a potential mechanism for Marcus Gunn Syndrome

• Published in: PloS one 2023-11, Vol.18 (11), p.e0293372-e0293372
• Main Authors: Shi, Xue, Zhang, Jingdong, Shi, Gaiping, Zhu, Junyi
• Format: Article
• Language: English
• Subjects: Amphibians; Analysis; Biology and Life Sciences; Care and treatment; Conjunctiva; Eye; Eyelid; Eyelid diseases; Fluorescence; Genetic factors; Hypotheses; Laboratory animals; Masseter muscle; Medicine and Health Sciences; Motor neurons; Muscles; Nerves; Neurons; Oculomotor nucleus; Patient outcomes; Prey; Sensory neurons; Surgery; Tracers; Tracers (Biology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0293372

Poking palpebral conjunctiva evoked upper-eyelid retraction during ophthalmic surgery. Iatrogenic eyelid ptosis occurred if eyelid branch of lachrymal nerve was sectioned. Mesencephalic trigeminal nucleus (Vme) neurons were labeled when tracer injected into lachrymal nerve innervating eyelid Mueller's muscle. Masseter afferent Vme neurons projecting to oculomotor nucleus (III) was observed in toad and rat, which helps amphibians to stare prey when they open mouth widely to prey. We hypothesized single Vme neurons may have peripheral collaterals to both eyelid and masseter muscles. WGA-594 was injected into upper eyelid, and WGA-488 was simultaneously delivered into ipsilateral masseter muscle in the same rat. Then, double labeled Vme neurons were found under both conventional and confocal microscope. Meanwhile, contact of WGA-594 positive eyelid afferent Vme neurons with WGA-488 labeled masseter afferent ones were observed sometimes. Combined with our previous observation of oculomotor projection Vme neurons, we thought WGA-594/488 double labeled Vme cells, at least some of them, are oculomotor projecting ones. Contact between eyelid and masseter afferent Vme neurons are supposed to be electrotonically coupled, based on a line of previous studies. If exogenous or genetic factors make these Vme neurons misinterpret masseter input as eyelid afferent signals, these Vme neurons might feedforward massages to eyelid retractor motoneurons in the III. Besides, oculomotor projecting Vme neurons might be co-fired by adjacent masseter afferent Vme neurons through electrotonic coupling once the masseter muscle is activated. In these cases, Marcus Gunn Syndrome might occur. This finding leads to a new hypothesis for the Syndrome.