Bacterial production of ciprofloxacin and potential usage as a radiotracer

Infectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave m...

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Published in:PloS one 2023-11, Vol.18 (11), p.e0291342-e0291342
Main Authors: Karatay, Kadriye Busra, Dogruoz Gungor, Nihal, Colak, Batu, Biber Muftuler, Fazilet Zumrut, Aras, Omer
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacteria
Bacterial diseases
Bacterial infections
Biology and Life Sciences
Care and treatment
Chromatography
Ciprofloxacin
Ciprofloxacin - chemistry
Ciprofloxacin - pharmacology
Diagnosis
Drug resistance
E coli
Escherichia coli - metabolism
Evaluation
Gas flow
High performance liquid chromatography
Infectious diseases
Liquid chromatography
Medical diagnosis
Medical imaging
Medical research
Medicine and Health Sciences
Methicillin
Methods
Microorganisms
Nuclear medicine
Peptides
Properties
Radioactive tracers
Radioisotopes
Radiolabelling
Radionuclide Imaging
Radiopharmaceuticals - chemistry
Research and Analysis Methods
Sinkholes
Staphylococcus infections
Technetium
Technetium - chemistry
Technetium isotopes
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creator Karatay, Kadriye Busra
Dogruoz Gungor, Nihal
Colak, Batu
Biber Muftuler, Fazilet Zumrut
Aras, Omer
description Infectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole," (Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide.
doi_str_mv 10.1371/journal.pone.0291342
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The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>37943851</pmid><doi>10.1371/journal.pone.0291342</doi><tpages>e0291342</tpages><orcidid>https://orcid.org/0000-0001-8184-9660</orcidid><orcidid>https://orcid.org/0000-0003-1758-0542</orcidid><oa>free_for_read</oa></addata></record>
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subjects Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacteria
Bacterial diseases
Bacterial infections
Biology and Life Sciences
Care and treatment
Chromatography
Ciprofloxacin
Ciprofloxacin - chemistry
Ciprofloxacin - pharmacology
Diagnosis
Drug resistance
E coli
Escherichia coli - metabolism
Evaluation
Gas flow
High performance liquid chromatography
Infectious diseases
Liquid chromatography
Medical diagnosis
Medical imaging
Medical research
Medicine and Health Sciences
Methicillin
Methods
Microorganisms
Nuclear medicine
Peptides
Properties
Radioactive tracers
Radioisotopes
Radiolabelling
Radionuclide Imaging
Radiopharmaceuticals - chemistry
Research and Analysis Methods
Sinkholes
Staphylococcus infections
Technetium
Technetium - chemistry
Technetium isotopes
title Bacterial production of ciprofloxacin and potential usage as a radiotracer
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