Age and sex-related variations in murine laryngeal microbiota

The larynx undergoes significant age and sex-related changes in structure and function across the lifespan. Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiologi...

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Published in:PloS one 2024-05, Vol.19 (5), p.e0300672-e0300672
Main Authors: An, Ran, Venkatraman, Anumitha, Binns, John, Saric, Callie, Rey, Federico E, Thibeault, Susan L
Format: Article
Language:English
Subjects:
Adolescents
Adults
Age
Age Factors
Age groups
Aging - physiology
Animal models
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Biological diversity
Biology and Life Sciences
Comparative analysis
Environmental effects
Ethylenediaminetetraacetic acid
Fatty acids
Female
Fourier transforms
Gender differences
Gene amplification
Gene sequencing
Genetic testing
Immunology
Immunoregulation
Intestinal microflora
Larynx
Larynx - microbiology
Life span
Male
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Microbiomes
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Older people
Pathogenesis
Pathology
People and Places
Physiological aspects
Physiology
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sex
Sex Factors
Sexes
Structure-function relationships
Variance analysis
Variation
Young adults
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Venkatraman, Anumitha
Binns, John
Saric, Callie
Rey, Federico E
Thibeault, Susan L
description The larynx undergoes significant age and sex-related changes in structure and function across the lifespan. Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. There was an abundance of short-chain fatty acid producing bacteria in the adolescent group, unique to the laryngeal microbiota; taxonomic changes in the elderly resembled that of the aged gut microbiome. There were no significant changes in the laryngeal microbiota relating to biological sex. This is the first study to report age and sex-related variation in laryngeal microbiota. This data lays the groundwork for defining how age-related microbial mechanisms may govern laryngeal health and disease. Bacterial compositional changes, as a result of environmental or systemic stimuli, may not only be indicative of laryngeal-specific metabolic and immunoregulatory processes, but may precede structural and functional age-related changes in laryngeal physiology.
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Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. 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source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central
subjects Adolescents
Adults
Age
Age Factors
Age groups
Aging - physiology
Animal models
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Biological diversity
Biology and Life Sciences
Comparative analysis
Environmental effects
Ethylenediaminetetraacetic acid
Fatty acids
Female
Fourier transforms
Gender differences
Gene amplification
Gene sequencing
Genetic testing
Immunology
Immunoregulation
Intestinal microflora
Larynx
Larynx - microbiology
Life span
Male
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Microbiomes
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Older people
Pathogenesis
Pathology
People and Places
Physiological aspects
Physiology
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sex
Sex Factors
Sexes
Structure-function relationships
Variance analysis
Variation
Young adults
title Age and sex-related variations in murine laryngeal microbiota
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