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Examining the influence of environmental factors on Acanthamoeba castellanii and Pseudomonas aeruginosa in co-culture
Exploration of interspecies interactions between microorganisms can have taxonomic, ecological, evolutionary, or medical applications. To better explore interactions between microorganisms it is important to establish the ideal conditions that ensure survival of all species involved. In this study,...
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description | Exploration of interspecies interactions between microorganisms can have taxonomic, ecological, evolutionary, or medical applications. To better explore interactions between microorganisms it is important to establish the ideal conditions that ensure survival of all species involved. In this study, we sought to identify the ideal biotic and abiotic factors that would result in high co-culture viability of two interkingdom species, Pseudomonas aeruginosa and Acanthamoeba castellanii, two soil dwelling microbes. There have been limited studies showing long-term interactions between these two organisms as co-culture can result in high mortality for one or both organisms suggesting a predator-predator interaction may exist between them. In this study, we identified biotic and abiotic conditions that resulted in a high viability for both organisms in long-term co-culture, including optimizing temperature, nutrient concentration, choice of bacterial strains, and the initial ratio of interacting partners. These two species represent ideal partners for studying microbial interactions because amoebae act similarly to mammalian immune cells in many respects, and this can allow researchers to study host-pathogen interactions in vitro. Therefore, long-term interaction studies between these microbes might reveal the evolutionary steps that occur in bacteria when subjected to intense predation, like what occurs when pathogens enter the human body. The culture conditions characterized here resulted in high viability for both organisms for at least 14-days in co-culture suggesting that long-term experimental studies between these species can be achieved using these culture conditions. |
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To better explore interactions between microorganisms it is important to establish the ideal conditions that ensure survival of all species involved. In this study, we sought to identify the ideal biotic and abiotic factors that would result in high co-culture viability of two interkingdom species, Pseudomonas aeruginosa and Acanthamoeba castellanii, two soil dwelling microbes. There have been limited studies showing long-term interactions between these two organisms as co-culture can result in high mortality for one or both organisms suggesting a predator-predator interaction may exist between them. In this study, we identified biotic and abiotic conditions that resulted in a high viability for both organisms in long-term co-culture, including optimizing temperature, nutrient concentration, choice of bacterial strains, and the initial ratio of interacting partners. These two species represent ideal partners for studying microbial interactions because amoebae act similarly to mammalian immune cells in many respects, and this can allow researchers to study host-pathogen interactions in vitro. Therefore, long-term interaction studies between these microbes might reveal the evolutionary steps that occur in bacteria when subjected to intense predation, like what occurs when pathogens enter the human body. The culture conditions characterized here resulted in high viability for both organisms for at least 14-days in co-culture suggesting that long-term experimental studies between these species can be achieved using these culture conditions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0305973</identifier><identifier>PMID: 38913685</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic factors ; Acanthamoeba castellanii ; Acanthamoeba castellanii - microbiology ; Bacteria ; Biology and Life Sciences ; Cell culture ; Coculture Techniques ; Culture ; Cysts ; Encephalitis ; Environmental factors ; Experiments ; Genomes ; Host-pathogen interactions ; Immune system ; Kinases ; Medicine and Health Sciences ; Microbial Interactions ; Microorganisms ; Nosocomial infections ; Nutrient concentrations ; Pathogens ; Physical Sciences ; Predation ; Predators ; Proteins ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - physiology ; Research and Analysis Methods ; Soil Microbiology ; Temperature ; Viability ; Virulence</subject><ispartof>PloS one, 2024-06, Vol.19 (6), p.e0305973</ispartof><rights>Copyright: © 2024 Cecil, Yoder-Himes. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>2024 Cecil, Yoder-Himes. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Cecil, Yoder-Himes 2024 Cecil, Yoder-Himes</rights><rights>2024 Cecil, Yoder-Himes. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c406t-b925e26098ee98940a64bed8e643c25e6896c3b4768cf67c0608f07487a06e2e3</cites><orcidid>0000-0001-8744-3194 ; 0000-0001-9576-3833</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3071893589/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3071893589?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38913685$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Smith, Robert P.</contributor><creatorcontrib>Cecil, Rhiannon E</creatorcontrib><creatorcontrib>Yoder-Himes, Deborah R</creatorcontrib><title>Examining the influence of environmental factors on Acanthamoeba castellanii and Pseudomonas aeruginosa in co-culture</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Exploration of interspecies interactions between microorganisms can have taxonomic, ecological, evolutionary, or medical applications. To better explore interactions between microorganisms it is important to establish the ideal conditions that ensure survival of all species involved. In this study, we sought to identify the ideal biotic and abiotic factors that would result in high co-culture viability of two interkingdom species, Pseudomonas aeruginosa and Acanthamoeba castellanii, two soil dwelling microbes. There have been limited studies showing long-term interactions between these two organisms as co-culture can result in high mortality for one or both organisms suggesting a predator-predator interaction may exist between them. In this study, we identified biotic and abiotic conditions that resulted in a high viability for both organisms in long-term co-culture, including optimizing temperature, nutrient concentration, choice of bacterial strains, and the initial ratio of interacting partners. These two species represent ideal partners for studying microbial interactions because amoebae act similarly to mammalian immune cells in many respects, and this can allow researchers to study host-pathogen interactions in vitro. Therefore, long-term interaction studies between these microbes might reveal the evolutionary steps that occur in bacteria when subjected to intense predation, like what occurs when pathogens enter the human body. The culture conditions characterized here resulted in high viability for both organisms for at least 14-days in co-culture suggesting that long-term experimental studies between these species can be achieved using these culture conditions.</description><subject>Abiotic factors</subject><subject>Acanthamoeba castellanii</subject><subject>Acanthamoeba castellanii - microbiology</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Cell culture</subject><subject>Coculture Techniques</subject><subject>Culture</subject><subject>Cysts</subject><subject>Encephalitis</subject><subject>Environmental factors</subject><subject>Experiments</subject><subject>Genomes</subject><subject>Host-pathogen interactions</subject><subject>Immune system</subject><subject>Kinases</subject><subject>Medicine and Health Sciences</subject><subject>Microbial Interactions</subject><subject>Microorganisms</subject><subject>Nosocomial infections</subject><subject>Nutrient concentrations</subject><subject>Pathogens</subject><subject>Physical Sciences</subject><subject>Predation</subject><subject>Predators</subject><subject>Proteins</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - 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To better explore interactions between microorganisms it is important to establish the ideal conditions that ensure survival of all species involved. In this study, we sought to identify the ideal biotic and abiotic factors that would result in high co-culture viability of two interkingdom species, Pseudomonas aeruginosa and Acanthamoeba castellanii, two soil dwelling microbes. There have been limited studies showing long-term interactions between these two organisms as co-culture can result in high mortality for one or both organisms suggesting a predator-predator interaction may exist between them. In this study, we identified biotic and abiotic conditions that resulted in a high viability for both organisms in long-term co-culture, including optimizing temperature, nutrient concentration, choice of bacterial strains, and the initial ratio of interacting partners. 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subjects | Abiotic factors Acanthamoeba castellanii Acanthamoeba castellanii - microbiology Bacteria Biology and Life Sciences Cell culture Coculture Techniques Culture Cysts Encephalitis Environmental factors Experiments Genomes Host-pathogen interactions Immune system Kinases Medicine and Health Sciences Microbial Interactions Microorganisms Nosocomial infections Nutrient concentrations Pathogens Physical Sciences Predation Predators Proteins Pseudomonas aeruginosa Pseudomonas aeruginosa - physiology Research and Analysis Methods Soil Microbiology Temperature Viability Virulence |
title | Examining the influence of environmental factors on Acanthamoeba castellanii and Pseudomonas aeruginosa in co-culture |
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