Metagenomic discovery of lipases with predicted structural similarity to Candida antarctica lipase B

Here we employed sequence-based and structure-based screening for prospecting lipases that have structural homolog to Candida antarctica lipase B (CalB). CalB, a widely used biocatalyst, was used as structural template reference because of its enzymatic properties. Structural homolog could aid in th...

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Bibliographic Details
Published in:PloS one 2023-12, Vol.18 (12), p.e0295397-e0295397
Main Authors: Jaito, Nongluck, Kaewsawat, Nattha, Phetlum, Suthathip, Uengwetwanit, Tanaporn
Format: Article
Language:English
Subjects:
Analysis
Antifungal agents
Biocatalysts
Bioprospecting
Biotechnology
Candida
Candida antarctica
E coli
Enzymes
Enzymes, Immobilized - chemistry
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes
Genomes
Health aspects
Lipase
Lipase - chemistry
Lipase - genetics
Maximum likelihood method
Metagenomics
pH effects
Phylogenetics
Plasmids
Proteins
Similarity
Simulation
Temperature
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Summary:Here we employed sequence-based and structure-based screening for prospecting lipases that have structural homolog to Candida antarctica lipase B (CalB). CalB, a widely used biocatalyst, was used as structural template reference because of its enzymatic properties. Structural homolog could aid in the discovery of novel wild-type enzymes with desirable features and serve as a scaffold for further biocatalyst design. The available metagenomic data isolated from various environments was leveraged as a source for bioprospecting. We identified two bacteria lipases that showed high structural similarity to CalB with
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0295397