Dietary magnesium, C-reactive protein and interleukin-6: The Strong Heart Family Study

• Published in: PloS one 2023-12, Vol.18 (12), p.e0296238
• Main Authors: Rao, Nandana D, Lemaitre, Rozenn N, Sitlani, Colleen M, Umans, Jason G, Haack, Karin, Handeland, Veronica, Navas-Acien, Ana, Cole, Shelley A, Best, Lyle G, Fretts, Amanda M
• Format: Article
• Language: English
• Subjects: Biology and Life Sciences; Biomarkers; Body mass index; C-reactive protein; C-Reactive Protein - metabolism; Calories; Cardiovascular disease; Cross-Sectional Studies; Cytokines; Data collection; Diabetes; Diet; Dietary intake; Dietary supplements; Family; Family studies; Food habits; Food intake; Gene polymorphism; Genetic aspects; Genotype & phenotype; Health aspects; Heart; Humans; Inflammation; Interleukin 6; Interleukin-6 - genetics; Interleukins; Magnesium; Magnesium in the body; Medicine and Health Sciences; Metabolism; Native Americans; Nucleotides; Nutrition research; Physiological aspects; Polymorphism; Proteins; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Surveys
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0296238

To examine the associations of dietary Mg intake with inflammatory biomarkers (C-reactive protein (CRP) and interleukin 6 (IL-6)), and the interaction of dietary Mg intake with single nucleotide polymorphism (SNP) rs3740393, a SNP related to Mg metabolism and transport, on CRP and IL-6 among American Indians (AIs). This cross-sectional study included AI participants (n = 1,924) from the Strong Heart Family Study (SHFS). Mg intake from foods and dietary supplements was ascertained using a 119-item Block food frequency questionnaire, CRP and IL-6 were measured from blood, and SNP rs3740393 was genotyped using MetaboChip. Generalized estimating equations were used to examine associations of Mg intake, and the interaction between rs3740393 and dietary Mg, with CRP and IL-6. Reported Mg intake was not associated with CRP or IL-6, irrespective of genotype. A significant interaction (p-interaction = 0.018) was observed between Mg intake and rs3740393 on IL-6. Among participants with the C/C genotype, for every 1 SD higher in log-Mg, log-IL-6 was 0.04 (95% CI: -0.10 to 0.17) pg/mL higher. Among participants with the C/G genotype, for every 1 SD higher in log-Mg, log-IL-6 was 0.08 (95% CI: -0.21 to 0.05) pg/mL lower, and among participants with the G/G genotype, for every 1 SD higher in log-Mg, log-IL-6 was 0.19 (95% CI: -0.38 to -0.01) pg/mL lower. Mg intake may be associated with lower IL-6 with increasing dosage of the G allele at rs3740393. Future research is necessary to replicate this finding and examine other Mg-related genes that influence associations of Mg intake with inflammation.