Evaluating the transmission dynamics and host competency of aoudad (Ammotragus lervia) experimentally infected with Mycoplasma ovipneumoniae and leukotoxigenic Pasteurellaceae

• Published in: PloS one 2024-07, Vol.19 (7), p.e0294853
• Main Authors: Thomas, Logan F, Clontz, Dallas, Nunez, Chase M, Dittmar, Robert O, Hernandez, Froylán, Rech, Raquel R, Cook, Walter E
• Format: Article
• Language: English
• Subjects: Ammotragus lervia; Animal populations; Animals; Bacteria; Biology and Life Sciences; Bronchopneumonia; Captivity; Chronic infection; Development and progression; Disease control; Disease transmission; Euthanasia; Experimental infection; Female; Feral populations; Medical research; Medicine and Health Sciences; Mycoplasma ovipneumoniae; Mycoplasma ovipneumoniae - pathogenicity; Nalbuphine; Native species; Ovis canadensis; Pasteurellaceae; Pasteurellaceae - pathogenicity; Pasteurellaceae Infections - microbiology; Pasteurellaceae Infections - transmission; Pasteurellaceae Infections - veterinary; Pathogenicity; Pathogens; Pathological effects; Penicillin G; People and places; Pneumonia; Pneumonia, Mycoplasma - microbiology; Pneumonia, Mycoplasma - transmission; Pneumonia, Mycoplasma - veterinary; Population studies; Populations; Research parks; Respiratory diseases; Ruminants - microbiology; Seroconversion; Shedding; Sheep; Sheep Diseases - microbiology; Sheep Diseases - transmission; Sheep, Bighorn - microbiology; Sympatric populations
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0294853

Feral populations of aoudad (Ammotragus lervia) occur in Texas bighorn sheep (Ovis canadensis) habitat and pose several conceptual ecological threats to bighorn sheep re-establishment efforts. The potential threat of disease transmission from aoudad to bighorn sheep may exacerbate these issues, but the host competency of aoudad and subsequent pathophysiology and transmissibility of pneumonic pathogens involved in the bighorn sheep respiratory disease complex is largely unknown. Because the largest population-limiting diseases of bighorn sheep involve pathogens causing bronchopneumonia, we evaluated the host competency of aoudad for Mycoplasma ovipneumoniae and leukotoxigenic Pasteurellaceae. Specifically, we described the shedding dynamics, pathogen carriage, seroconversion, clinical patterns, and pathological effects of experimental infection among wild aoudad held in captivity. We found that aoudad are competent hosts capable of maintaining and intraspecifically transmitting Mycoplasma ovipneumoniae and Pasteurellaceae and can shed the bacteria for 53 days after exposure. Aoudad developed limited clinical signs and pathological findings ranged from mild chronic lymphohistiocytic bronchointerstitial pneumonia to severe and acute suppurative pneumonia, similarly, observed in bighorn sheep infected with Mycoplasma spp. and Pasteurellaceae bacteria, respectively. Furthermore, as expected, clinical signs and lesions were often more severe in aoudad inoculated with a combination of Mycoplasma ovipneumoniae and Pasteurellaceae as compared to aoudad inoculated with only Mycoplasma ovipneumoniae. There may be evidence of interindividual susceptibility, pathogenicity, and/or transmissibility, indicated by individual aoudad maintaining varying severities of chronic infection who may be carriers continuously shedding pathogens. This is the first study to date to demonstrate that aoudad are a conceptual disease transmission threat to sympatric bighorn sheep populations due to their host competency and intraspecific transmission capabilities.