Extended-Spectrum β-Lactamase and carbapenemase-producing Escherichia coli O157:H7 among diarrheic patients in Shashemene, Ethiopia

• Published in: PloS one 2024-08, Vol.19 (8), p.e0306691
• Main Authors: Ayalneh, Shimelis Teshome, Beshah, Biruk Yeshitela, Jeon, Yeonji, Teshome, Seifegebriel, Getahun, Tomas, Gebreselassie, Solomon, Park, Se Eun, Teferi, Mekonnen, Abegaz, Woldaregay Erku
• Format: Article
• Language: English
• Subjects: Acid resistance; Adolescent; Adult; Amoxicillin; Ampicillin; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Antimicrobial agents; Antisera; Bacteria; Bacterial Proteins - metabolism; beta-Lactamases - metabolism; Biochemical tests; Biology and Life Sciences; Carbapenemase; Carbapenems; Cattle; Child; Child, Preschool; Ciprofloxacin; Clavulanic acid; Confidence intervals; Dairy products; Developing countries; Diarrhea - microbiology; Domestic animals; Drug resistance; E coli; Escherichia coli; Escherichia coli Infections - drug therapy; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli O157 - drug effects; Escherichia coli O157 - enzymology; Escherichia coli O157 - isolation & purification; Ethiopia - epidemiology; Family size; Feces - microbiology; Female; Health facilities; High resistance; Humans; Inactivation; Infant; Lactose; LDCs; Male; Medicine and Health Sciences; Microbial Sensitivity Tests; Middle Aged; Multidrug resistance; Multidrug resistant organisms; Pathogens; People and Places; Public health; Regression models; Research and Analysis Methods; Sample size; Samples; Sorbitol; Statistical analysis; Statistical models; Statistical significance; Urban areas; Young Adult; β Lactamase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0306691

The worldwide increase in multidrug resistance is a major threat to public health. One particular concern is the presence of Escherichia coli strains that carry Extended-Spectrum β-Lactamase (ESBL) and Carbapenemase enzymes, which can make multiple antibiotics ineffective. This complicates treatment strategies and raises the risk of illness and death. The aim of this study was to isolate E. coli O157:H7, assess its susceptibility against antimicrobial agents, and determine the presence of ESBL and Carbapenemase production in stool samples collected from diarrheic patients in Shashemene, west Arsi, Ethiopia from July to November 2022. The samples were cultured McConkey Agar and E. coli were isolated and identified by standard biochemical tests using API 20E. E. coli O157:H7 was further identified using sorbitol McConkey Agar and antisera for O157 antigen test. The antimicrobial susceptibility test was performed using the Kirby-Bauer disc diffusion method using different antibiotics. Each identified isolate was screened and tested for phenotypical ESBL and Carbapenemase production using combined disc method and modified carbapenem inactivation method, respectively. Bivariant and multivariant analyses were employed using a logistic regression model for further analysis and were interpreted based on the odds ratio and level of statistical significance at a p-value