An analysis of differential gene expression in peripheral nerve and muscle utilizing RNA sequencing after polyethylene glycol nerve fusion in a rat sciatic nerve injury model

Application of polyethylene glycol (PEG) to a peripheral nerve injury at the time of primary neurorrhaphy is thought to prevent Wallerian degeneration via direct axolemma fusion. The molecular mechanisms of nerve fusion and recovery are unclear. Our study tested the hypothesis that PEG alters gene e...

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Bibliographic Details
Published in:PloS one 2024-09, Vol.19 (9), p.e0304773
Main Authors: Weiss, Samantha N, Legato, Joseph M, Liu, Yichuan, Vaccaro, Courtney N, Da Silva, Renata Pellegrino, Miskiel, Sandra, Gilbert, Grace V, Hakonarson, Hakon, Fuller, David A, Buono, Russell J
Format: Article
Language:English
Subjects:
Analysis
Animals
Bioinformatics
Biology and Life Sciences
Care and treatment
Clinical outcomes
Composition
Degeneration
Diagnosis
Disease Models, Animal
Gene expression
Gene Expression Profiling
Gene Expression Regulation - drug effects
Gene fusion
Gene regulation
Gene sequencing
Genetic aspects
Injuries
Injury analysis
Injury prevention
Innervation
Laboratory animals
Male
Medical examination
Medicine and Health Sciences
Molecular modelling
Muscle, Skeletal - drug effects
Muscle, Skeletal - innervation
Muscle, Skeletal - metabolism
Muscles
Nerve Regeneration - drug effects
Nerve Regeneration - genetics
Nervous system
Neurodegeneration
Patient satisfaction
Peripheral nerve diseases
Peripheral Nerve Injuries - genetics
Peripheral nerves
Polyethylene glycol
Polyethylene Glycols - pharmacology
Rats
Rats, Inbred Lew
Recovery
Recovery of function
Research and analysis methods
Ribonucleic acid
RNA
RNA sequencing
Sciatic nerve
Sciatic Nerve - injuries
Sequence Analysis, RNA
Sutures
Trauma
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Summary:Application of polyethylene glycol (PEG) to a peripheral nerve injury at the time of primary neurorrhaphy is thought to prevent Wallerian degeneration via direct axolemma fusion. The molecular mechanisms of nerve fusion and recovery are unclear. Our study tested the hypothesis that PEG alters gene expression in neural and muscular environments as part of its restorative properties. Lewis rats underwent unilateral sciatic nerve transection with immediate primary repair. Subjects were randomly assigned to receive either PEG treatment or standard repair at the time of neurorrhaphy. Samples of sciatic nerve distal to the injury and tibialis muscle at the site of innervation were harvested at 24 hours and 4 weeks postoperatively. Total RNA sequencing and subsequent bioinformatics analyses were used to identify significant differences in differentially expressed genes (DEGs) and their related biological pathways (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0304773