A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds

• Published in: PloS one 2024-09, Vol.19 (9), p.e0310336
• Main Authors: Harahap, Ratna Sholatia, Gunawan, Asep, Endrawati, Yuni Cahya, Darusman, Huda Shalahudin, Andersson, Göran, Noor, Ronny Rachman
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Amino acids; Analysis; Animal populations; Animals; Beef; Biology and Life Sciences; Breeding; Carcasses; Cholesterol; CYP2E1 gene; CYP2E1 protein; Cytochrome; Cytochrome P-450 CYP2E1 - genetics; Cytochrome P-450 CYP2E1 - metabolism; Diseases; Fatty acids; Flavors; Gene expression; Gene Frequency; Gene polymorphism; Genes; Genetic analysis; Genetic aspects; Genetic markers; Genotype; Genotypes; Growth; Halal food; Immunohistochemistry; Indonesia; Lamb; Meat; Meat - analysis; Meat quality; Medicine and Health Sciences; Metabolism; Muscles; Musculoskeletal system; Odor; Ovis aries; Oxidation; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Protein expression; Proteins; Quality management; R&D; Red Meat - analysis; Research & development; Research and Analysis Methods; Restriction fragment length polymorphism; RNA; Sheep; Sheep - genetics; Sheep - metabolism; Sheep breeds; Sheep, Domestic - genetics; Sheep, Domestic - metabolism; Single nucleotide polymorphisms; Single-nucleotide polymorphism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0310336

The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T>C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T>C CYP2E1 gene and lamb quality traits were significant (P