A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds

The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T>C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carc...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2024-09, Vol.19 (9), p.e0310336
Main Authors: Harahap, Ratna Sholatia, Gunawan, Asep, Endrawati, Yuni Cahya, Darusman, Huda Shalahudin, Andersson, Göran, Noor, Ronny Rachman
Format: Article
Language:English
Subjects:
3' Untranslated regions
Amino acids
Analysis
Animal populations
Animals
Beef
Biology and Life Sciences
Breeding
Carcasses
Cholesterol
CYP2E1 gene
CYP2E1 protein
Cytochrome
Cytochrome P-450 CYP2E1 - genetics
Cytochrome P-450 CYP2E1 - metabolism
Diseases
Fatty acids
Flavors
Gene expression
Gene Frequency
Gene polymorphism
Genes
Genetic analysis
Genetic aspects
Genetic markers
Genotype
Genotypes
Growth
Halal food
Immunohistochemistry
Indonesia
Lamb
Meat
Meat - analysis
Meat quality
Medicine and Health Sciences
Metabolism
Muscles
Musculoskeletal system
Odor
Ovis aries
Oxidation
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Protein expression
Proteins
Quality management
R&D
Red Meat - analysis
Research & development
Research and Analysis Methods
Restriction fragment length polymorphism
RNA
Sheep
Sheep - genetics
Sheep - metabolism
Sheep breeds
Sheep, Domestic - genetics
Sheep, Domestic - metabolism
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c506t-cb587673fd2889143d79a150be8a635630fc25b80c817a4b8a0fb79b1f7d75383
container_end_page
container_issue 9
container_start_page e0310336
container_title PloS one
container_volume 19
creator Harahap, Ratna Sholatia
Gunawan, Asep
Endrawati, Yuni Cahya
Darusman, Huda Shalahudin
Andersson, Göran
Noor, Ronny Rachman
description The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T>C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T>C CYP2E1 gene and lamb quality traits were significant (P
doi_str_mv 10.1371/journal.pone.0310336
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3102337597</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A807822870</galeid><sourcerecordid>A807822870</sourcerecordid><originalsourceid>FETCH-LOGICAL-c506t-cb587673fd2889143d79a150be8a635630fc25b80c817a4b8a0fb79b1f7d75383</originalsourceid><addsrcrecordid>eNqNkt-K1DAUxoso7rr6BqIBQfRixqSZNu2VDMOqAwsr_gOvQpqeTLOkyWySLs4z-NKmTneZkb2QXCQkv-87hy8ny54TPCeUkXdXbvBWmPnWWZhjSjCl5YPslNQ0n5U5pg8PzifZkxCuMC5oVZaPsxNa5wVe1OVp9nuJpOu3HjqwQd8ACnFod8gptPr5OT8nSNgW6RiQdwaQtkgKL0UISHbCCxnB6xC1DH852UGvpTDIiL5BPYiIrgdhdNyNylYrBR5sRGvbpqaDFhaFDmCLGg_QhqfZIyVMgGfTfpZ9_3D-bfVpdnH5cb1aXsxkgcs4k01RsZJR1eZVVZMFbVktSIEbqERJi5JiJfOiqbCsCBOLphJYNaxuiGItSwnQs-zl3ndrXOBTjoGnCHNKWVGzRLyfiKHpoZWpaS8M33rdC7_jTmh-_GJ1xzfuhhOS_HMy1ngzOXh3PUCIvNdBgjHCghv2xRZlXeZjsVf_oPe3NFEbYYBrq1wqLEdTvqwwq_K8YjhR83uotNrxZ1LoSqf7I8HbI0FiIvyKGzGEwNdfv_w_e_njmH19wHYgTOyCM0PUzoZjcLEHpXcheFB3KRPMx0G_TYOPg86nQU-yF4c_dCe6nWz6BwK7-O4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3102337597</pqid></control><display><type>article</type><title>A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Harahap, Ratna Sholatia ; Gunawan, Asep ; Endrawati, Yuni Cahya ; Darusman, Huda Shalahudin ; Andersson, Göran ; Noor, Ronny Rachman</creator><contributor>Mandal, Palash</contributor><creatorcontrib>Harahap, Ratna Sholatia ; Gunawan, Asep ; Endrawati, Yuni Cahya ; Darusman, Huda Shalahudin ; Andersson, Göran ; Noor, Ronny Rachman ; Mandal, Palash</creatorcontrib><description>The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T&gt;C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T&gt;C CYP2E1 gene and lamb quality traits were significant (P&lt;0.05), including carcass characteristics, retail cut carcass, fatty acid, cholesterol, flavor, and odor. Lambs with the CT genotype had a higher mRNA and protein expression in high lamb quality traits. The highest CYP2E1 protein expression was localized in the longissimus dorsi. The group sample with high lamb quality had a higher area and perimeter of muscle cells. CYP2E1 can be used as a genetic marker for selecting sheep with high meat quality.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0310336</identifier><identifier>PMID: 39250496</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>3' Untranslated regions ; Amino acids ; Analysis ; Animal populations ; Animals ; Beef ; Biology and Life Sciences ; Breeding ; Carcasses ; Cholesterol ; CYP2E1 gene ; CYP2E1 protein ; Cytochrome ; Cytochrome P-450 CYP2E1 - genetics ; Cytochrome P-450 CYP2E1 - metabolism ; Diseases ; Fatty acids ; Flavors ; Gene expression ; Gene Frequency ; Gene polymorphism ; Genes ; Genetic analysis ; Genetic aspects ; Genetic markers ; Genotype ; Genotypes ; Growth ; Halal food ; Immunohistochemistry ; Indonesia ; Lamb ; Meat ; Meat - analysis ; Meat quality ; Medicine and Health Sciences ; Metabolism ; Muscles ; Musculoskeletal system ; Odor ; Ovis aries ; Oxidation ; Polymerase chain reaction ; Polymorphism ; Polymorphism, Single Nucleotide ; Protein expression ; Proteins ; Quality management ; R&amp;D ; Red Meat - analysis ; Research &amp; development ; Research and Analysis Methods ; Restriction fragment length polymorphism ; RNA ; Sheep ; Sheep - genetics ; Sheep - metabolism ; Sheep breeds ; Sheep, Domestic - genetics ; Sheep, Domestic - metabolism ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism</subject><ispartof>PloS one, 2024-09, Vol.19 (9), p.e0310336</ispartof><rights>Copyright: © 2024 Harahap et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Harahap et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Harahap et al 2024 Harahap et al</rights><rights>2024 Harahap et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c506t-cb587673fd2889143d79a150be8a635630fc25b80c817a4b8a0fb79b1f7d75383</cites><orcidid>0000-0001-5246-4390 ; 0000-0001-8795-2992</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3102337597/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3102337597?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39250496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mandal, Palash</contributor><creatorcontrib>Harahap, Ratna Sholatia</creatorcontrib><creatorcontrib>Gunawan, Asep</creatorcontrib><creatorcontrib>Endrawati, Yuni Cahya</creatorcontrib><creatorcontrib>Darusman, Huda Shalahudin</creatorcontrib><creatorcontrib>Andersson, Göran</creatorcontrib><creatorcontrib>Noor, Ronny Rachman</creatorcontrib><title>A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T&gt;C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T&gt;C CYP2E1 gene and lamb quality traits were significant (P&lt;0.05), including carcass characteristics, retail cut carcass, fatty acid, cholesterol, flavor, and odor. Lambs with the CT genotype had a higher mRNA and protein expression in high lamb quality traits. The highest CYP2E1 protein expression was localized in the longissimus dorsi. The group sample with high lamb quality had a higher area and perimeter of muscle cells. CYP2E1 can be used as a genetic marker for selecting sheep with high meat quality.</description><subject>3' Untranslated regions</subject><subject>Amino acids</subject><subject>Analysis</subject><subject>Animal populations</subject><subject>Animals</subject><subject>Beef</subject><subject>Biology and Life Sciences</subject><subject>Breeding</subject><subject>Carcasses</subject><subject>Cholesterol</subject><subject>CYP2E1 gene</subject><subject>CYP2E1 protein</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Cytochrome P-450 CYP2E1 - metabolism</subject><subject>Diseases</subject><subject>Fatty acids</subject><subject>Flavors</subject><subject>Gene expression</subject><subject>Gene Frequency</subject><subject>Gene polymorphism</subject><subject>Genes</subject><subject>Genetic analysis</subject><subject>Genetic aspects</subject><subject>Genetic markers</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Growth</subject><subject>Halal food</subject><subject>Immunohistochemistry</subject><subject>Indonesia</subject><subject>Lamb</subject><subject>Meat</subject><subject>Meat - analysis</subject><subject>Meat quality</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Odor</subject><subject>Ovis aries</subject><subject>Oxidation</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Quality management</subject><subject>R&amp;D</subject><subject>Red Meat - analysis</subject><subject>Research &amp; development</subject><subject>Research and Analysis Methods</subject><subject>Restriction fragment length polymorphism</subject><subject>RNA</subject><subject>Sheep</subject><subject>Sheep - genetics</subject><subject>Sheep - metabolism</subject><subject>Sheep breeds</subject><subject>Sheep, Domestic - genetics</subject><subject>Sheep, Domestic - metabolism</subject><subject>Single nucleotide polymorphisms</subject><subject>Single-nucleotide polymorphism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkt-K1DAUxoso7rr6BqIBQfRixqSZNu2VDMOqAwsr_gOvQpqeTLOkyWySLs4z-NKmTneZkb2QXCQkv-87hy8ny54TPCeUkXdXbvBWmPnWWZhjSjCl5YPslNQ0n5U5pg8PzifZkxCuMC5oVZaPsxNa5wVe1OVp9nuJpOu3HjqwQd8ACnFod8gptPr5OT8nSNgW6RiQdwaQtkgKL0UISHbCCxnB6xC1DH852UGvpTDIiL5BPYiIrgdhdNyNylYrBR5sRGvbpqaDFhaFDmCLGg_QhqfZIyVMgGfTfpZ9_3D-bfVpdnH5cb1aXsxkgcs4k01RsZJR1eZVVZMFbVktSIEbqERJi5JiJfOiqbCsCBOLphJYNaxuiGItSwnQs-zl3ndrXOBTjoGnCHNKWVGzRLyfiKHpoZWpaS8M33rdC7_jTmh-_GJ1xzfuhhOS_HMy1ngzOXh3PUCIvNdBgjHCghv2xRZlXeZjsVf_oPe3NFEbYYBrq1wqLEdTvqwwq_K8YjhR83uotNrxZ1LoSqf7I8HbI0FiIvyKGzGEwNdfv_w_e_njmH19wHYgTOyCM0PUzoZjcLEHpXcheFB3KRPMx0G_TYOPg86nQU-yF4c_dCe6nWz6BwK7-O4</recordid><startdate>20240909</startdate><enddate>20240909</enddate><creator>Harahap, Ratna Sholatia</creator><creator>Gunawan, Asep</creator><creator>Endrawati, Yuni Cahya</creator><creator>Darusman, Huda Shalahudin</creator><creator>Andersson, Göran</creator><creator>Noor, Ronny Rachman</creator><general>Public Library of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5246-4390</orcidid><orcidid>https://orcid.org/0000-0001-8795-2992</orcidid></search><sort><creationdate>20240909</creationdate><title>A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds</title><author>Harahap, Ratna Sholatia ; Gunawan, Asep ; Endrawati, Yuni Cahya ; Darusman, Huda Shalahudin ; Andersson, Göran ; Noor, Ronny Rachman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-cb587673fd2889143d79a150be8a635630fc25b80c817a4b8a0fb79b1f7d75383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>3' Untranslated regions</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Animal populations</topic><topic>Animals</topic><topic>Beef</topic><topic>Biology and Life Sciences</topic><topic>Breeding</topic><topic>Carcasses</topic><topic>Cholesterol</topic><topic>CYP2E1 gene</topic><topic>CYP2E1 protein</topic><topic>Cytochrome</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Cytochrome P-450 CYP2E1 - metabolism</topic><topic>Diseases</topic><topic>Fatty acids</topic><topic>Flavors</topic><topic>Gene expression</topic><topic>Gene Frequency</topic><topic>Gene polymorphism</topic><topic>Genes</topic><topic>Genetic analysis</topic><topic>Genetic aspects</topic><topic>Genetic markers</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Growth</topic><topic>Halal food</topic><topic>Immunohistochemistry</topic><topic>Indonesia</topic><topic>Lamb</topic><topic>Meat</topic><topic>Meat - analysis</topic><topic>Meat quality</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>Odor</topic><topic>Ovis aries</topic><topic>Oxidation</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Quality management</topic><topic>R&amp;D</topic><topic>Red Meat - analysis</topic><topic>Research &amp; development</topic><topic>Research and Analysis Methods</topic><topic>Restriction fragment length polymorphism</topic><topic>RNA</topic><topic>Sheep</topic><topic>Sheep - genetics</topic><topic>Sheep - metabolism</topic><topic>Sheep breeds</topic><topic>Sheep, Domestic - genetics</topic><topic>Sheep, Domestic - metabolism</topic><topic>Single nucleotide polymorphisms</topic><topic>Single-nucleotide polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harahap, Ratna Sholatia</creatorcontrib><creatorcontrib>Gunawan, Asep</creatorcontrib><creatorcontrib>Endrawati, Yuni Cahya</creatorcontrib><creatorcontrib>Darusman, Huda Shalahudin</creatorcontrib><creatorcontrib>Andersson, Göran</creatorcontrib><creatorcontrib>Noor, Ronny Rachman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints database</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>PHMC-Proquest健康医学期刊库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harahap, Ratna Sholatia</au><au>Gunawan, Asep</au><au>Endrawati, Yuni Cahya</au><au>Darusman, Huda Shalahudin</au><au>Andersson, Göran</au><au>Noor, Ronny Rachman</au><au>Mandal, Palash</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-09-09</date><risdate>2024</risdate><volume>19</volume><issue>9</issue><spage>e0310336</spage><pages>e0310336-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T&gt;C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T&gt;C CYP2E1 gene and lamb quality traits were significant (P&lt;0.05), including carcass characteristics, retail cut carcass, fatty acid, cholesterol, flavor, and odor. Lambs with the CT genotype had a higher mRNA and protein expression in high lamb quality traits. The highest CYP2E1 protein expression was localized in the longissimus dorsi. The group sample with high lamb quality had a higher area and perimeter of muscle cells. CYP2E1 can be used as a genetic marker for selecting sheep with high meat quality.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39250496</pmid><doi>10.1371/journal.pone.0310336</doi><tpages>e0310336</tpages><orcidid>https://orcid.org/0000-0001-5246-4390</orcidid><orcidid>https://orcid.org/0000-0001-8795-2992</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2024-09, Vol.19 (9), p.e0310336
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_3102337597
source Publicly Available Content Database; PubMed Central
subjects 3' Untranslated regions
Amino acids
Analysis
Animal populations
Animals
Beef
Biology and Life Sciences
Breeding
Carcasses
Cholesterol
CYP2E1 gene
CYP2E1 protein
Cytochrome
Cytochrome P-450 CYP2E1 - genetics
Cytochrome P-450 CYP2E1 - metabolism
Diseases
Fatty acids
Flavors
Gene expression
Gene Frequency
Gene polymorphism
Genes
Genetic analysis
Genetic aspects
Genetic markers
Genotype
Genotypes
Growth
Halal food
Immunohistochemistry
Indonesia
Lamb
Meat
Meat - analysis
Meat quality
Medicine and Health Sciences
Metabolism
Muscles
Musculoskeletal system
Odor
Ovis aries
Oxidation
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Protein expression
Proteins
Quality management
R&D
Red Meat - analysis
Research & development
Research and Analysis Methods
Restriction fragment length polymorphism
RNA
Sheep
Sheep - genetics
Sheep - metabolism
Sheep breeds
Sheep, Domestic - genetics
Sheep, Domestic - metabolism
Single nucleotide polymorphisms
Single-nucleotide polymorphism
title A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T06%3A03%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20comprehensive%20study%20of%20CYP2E1%20and%20its%20role%20in%20carcass%20characteristics%20and%20chemical%20lamb%20meat%20quality%20in%20different%20Indonesian%20sheep%20breeds&rft.jtitle=PloS%20one&rft.au=Harahap,%20Ratna%20Sholatia&rft.date=2024-09-09&rft.volume=19&rft.issue=9&rft.spage=e0310336&rft.pages=e0310336-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0310336&rft_dat=%3Cgale_plos_%3EA807822870%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c506t-cb587673fd2889143d79a150be8a635630fc25b80c817a4b8a0fb79b1f7d75383%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3102337597&rft_id=info:pmid/39250496&rft_galeid=A807822870&rfr_iscdi=true