Loading…
Visualizing VDAC1 in live cells using a tetracysteine tag
The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 in living cells. To permit precise VDAC1 imaging, we utilized...
Saved in:
Published in: | PloS one 2024-10, Vol.19 (10), p.e0311107 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | cdi_FETCH-LOGICAL-c450t-1ca9d92f0e6e12b355a80036306c69762d3ba10f474dc78137bbe615f0e4f9e73 |
container_end_page | |
container_issue | 10 |
container_start_page | e0311107 |
container_title | PloS one |
container_volume | 19 |
creator | Pilic, Johannes Oflaz, Furkan E Gottschalk, Benjamin Erdogan, Yusuf C Graier, Wolfgang F Malli, Roland |
description | The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 in living cells. To permit precise VDAC1 imaging, we utilized the tetracysteine (TC)-tag and visualized VDAC1 dynamics in living cells. TC-tagged VDAC1 had a cluster-like distribution on mitochondria. The labeling of TC-tagged VDAC1 was validated with immunofluorescence. The majority of VDAC1-TC-clusters were localized at endoplasmic reticulum (ER)-mitochondria contact sites. Notably, VDAC1 colocalized with BCL-2 Antagonist/Killer (BAK)-clusters upon apoptotic stimulation. Using this new tool, we were able to observe VDAC1-TC at mitochondrial fission sites. These findings highlight the suitability of the TC-tag for live-cell imaging of VDAC1, shedding light on the roles of VDAC1 in cellular processes. |
doi_str_mv | 10.1371/journal.pone.0311107 |
format | article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3118206928</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A812727946</galeid><sourcerecordid>A812727946</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-1ca9d92f0e6e12b355a80036306c69762d3ba10f474dc78137bbe615f0e4f9e73</originalsourceid><addsrcrecordid>eNqNkltr3DAQhUVpaS7NPwipoVDah93qYkvW47K9BQKBttlXIdvjjYLW2nrkkOTXV2adki15KHqQkL4zzBkdQk4ZnTOh2KebMPSd9fNt6GBOBWOMqhfkkGnBZ5JT8fLJ-YAcId5QWohSytfkQOicC87kIdErh4P17sF162z1ebFkmesy724hq8F7zAYcX2wWIfa2vscIroMs2vUb8qq1HuFk2o_J1dcvv5bfZxeX386Xi4tZnRc0zlhtdaN5S0EC45UoCltSKqSgspZaSd6IyjLa5ipvalUma1UFkhVJkLcalDgmb3d1tz6gmVyjSYZLTqXmZSI-TEQffg-A0Wwcjt3bDsKwQwXNmdIJffcP-nzBiVpbD8Z1bRi9j0XNomRccaVzmaj5M1RaDWxcnX6ldel-T_BxT5CYCHdxbQdEc_7zx_-zl6t99v0T9hqsj9cY_BBd6HAfzHdg3QfEHlqz7d3G9veGUTOG6nEaZgyVmUKVZGfT0IZqA81f0WOKxB-7BsMe</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3118206928</pqid></control><display><type>article</type><title>Visualizing VDAC1 in live cells using a tetracysteine tag</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central Free</source><creator>Pilic, Johannes ; Oflaz, Furkan E ; Gottschalk, Benjamin ; Erdogan, Yusuf C ; Graier, Wolfgang F ; Malli, Roland</creator><contributor>Kahle, Philipp J.</contributor><creatorcontrib>Pilic, Johannes ; Oflaz, Furkan E ; Gottschalk, Benjamin ; Erdogan, Yusuf C ; Graier, Wolfgang F ; Malli, Roland ; Kahle, Philipp J.</creatorcontrib><description>The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 in living cells. To permit precise VDAC1 imaging, we utilized the tetracysteine (TC)-tag and visualized VDAC1 dynamics in living cells. TC-tagged VDAC1 had a cluster-like distribution on mitochondria. The labeling of TC-tagged VDAC1 was validated with immunofluorescence. The majority of VDAC1-TC-clusters were localized at endoplasmic reticulum (ER)-mitochondria contact sites. Notably, VDAC1 colocalized with BCL-2 Antagonist/Killer (BAK)-clusters upon apoptotic stimulation. Using this new tool, we were able to observe VDAC1-TC at mitochondrial fission sites. These findings highlight the suitability of the TC-tag for live-cell imaging of VDAC1, shedding light on the roles of VDAC1 in cellular processes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0311107</identifier><identifier>PMID: 39423216</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibodies ; Apoptosis ; Bcl-2 protein ; Cell culture ; Cells ; Cells (biology) ; Clusters ; Cysteine ; Cysteine - metabolism ; Endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; Energy balance ; Glucose ; HeLa Cells ; Homeostasis ; Humans ; Imaging systems ; Immunofluorescence ; Ion channels ; Lasers ; Localization ; Methods ; Mitochondria ; Mitochondria - metabolism ; Mitochondrial Dynamics ; Morphology ; Penicillin ; Physiological aspects ; Proteins ; Voltage-Dependent Anion Channel 1 - metabolism</subject><ispartof>PloS one, 2024-10, Vol.19 (10), p.e0311107</ispartof><rights>Copyright: © 2024 Pilic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Pilic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Pilic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c450t-1ca9d92f0e6e12b355a80036306c69762d3ba10f474dc78137bbe615f0e4f9e73</cites><orcidid>0000-0001-6327-8729 ; 0000-0003-1871-3298</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3118206928/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3118206928?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39423216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kahle, Philipp J.</contributor><creatorcontrib>Pilic, Johannes</creatorcontrib><creatorcontrib>Oflaz, Furkan E</creatorcontrib><creatorcontrib>Gottschalk, Benjamin</creatorcontrib><creatorcontrib>Erdogan, Yusuf C</creatorcontrib><creatorcontrib>Graier, Wolfgang F</creatorcontrib><creatorcontrib>Malli, Roland</creatorcontrib><title>Visualizing VDAC1 in live cells using a tetracysteine tag</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 in living cells. To permit precise VDAC1 imaging, we utilized the tetracysteine (TC)-tag and visualized VDAC1 dynamics in living cells. TC-tagged VDAC1 had a cluster-like distribution on mitochondria. The labeling of TC-tagged VDAC1 was validated with immunofluorescence. The majority of VDAC1-TC-clusters were localized at endoplasmic reticulum (ER)-mitochondria contact sites. Notably, VDAC1 colocalized with BCL-2 Antagonist/Killer (BAK)-clusters upon apoptotic stimulation. Using this new tool, we were able to observe VDAC1-TC at mitochondrial fission sites. These findings highlight the suitability of the TC-tag for live-cell imaging of VDAC1, shedding light on the roles of VDAC1 in cellular processes.</description><subject>Antibodies</subject><subject>Apoptosis</subject><subject>Bcl-2 protein</subject><subject>Cell culture</subject><subject>Cells</subject><subject>Cells (biology)</subject><subject>Clusters</subject><subject>Cysteine</subject><subject>Cysteine - metabolism</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Energy balance</subject><subject>Glucose</subject><subject>HeLa Cells</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Imaging systems</subject><subject>Immunofluorescence</subject><subject>Ion channels</subject><subject>Lasers</subject><subject>Localization</subject><subject>Methods</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial Dynamics</subject><subject>Morphology</subject><subject>Penicillin</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Voltage-Dependent Anion Channel 1 - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkltr3DAQhUVpaS7NPwipoVDah93qYkvW47K9BQKBttlXIdvjjYLW2nrkkOTXV2adki15KHqQkL4zzBkdQk4ZnTOh2KebMPSd9fNt6GBOBWOMqhfkkGnBZ5JT8fLJ-YAcId5QWohSytfkQOicC87kIdErh4P17sF162z1ebFkmesy724hq8F7zAYcX2wWIfa2vscIroMs2vUb8qq1HuFk2o_J1dcvv5bfZxeX386Xi4tZnRc0zlhtdaN5S0EC45UoCltSKqSgspZaSd6IyjLa5ipvalUma1UFkhVJkLcalDgmb3d1tz6gmVyjSYZLTqXmZSI-TEQffg-A0Wwcjt3bDsKwQwXNmdIJffcP-nzBiVpbD8Z1bRi9j0XNomRccaVzmaj5M1RaDWxcnX6ldel-T_BxT5CYCHdxbQdEc_7zx_-zl6t99v0T9hqsj9cY_BBd6HAfzHdg3QfEHlqz7d3G9veGUTOG6nEaZgyVmUKVZGfT0IZqA81f0WOKxB-7BsMe</recordid><startdate>20241018</startdate><enddate>20241018</enddate><creator>Pilic, Johannes</creator><creator>Oflaz, Furkan E</creator><creator>Gottschalk, Benjamin</creator><creator>Erdogan, Yusuf C</creator><creator>Graier, Wolfgang F</creator><creator>Malli, Roland</creator><general>Public Library of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6327-8729</orcidid><orcidid>https://orcid.org/0000-0003-1871-3298</orcidid></search><sort><creationdate>20241018</creationdate><title>Visualizing VDAC1 in live cells using a tetracysteine tag</title><author>Pilic, Johannes ; Oflaz, Furkan E ; Gottschalk, Benjamin ; Erdogan, Yusuf C ; Graier, Wolfgang F ; Malli, Roland</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-1ca9d92f0e6e12b355a80036306c69762d3ba10f474dc78137bbe615f0e4f9e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibodies</topic><topic>Apoptosis</topic><topic>Bcl-2 protein</topic><topic>Cell culture</topic><topic>Cells</topic><topic>Cells (biology)</topic><topic>Clusters</topic><topic>Cysteine</topic><topic>Cysteine - metabolism</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Energy balance</topic><topic>Glucose</topic><topic>HeLa Cells</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Imaging systems</topic><topic>Immunofluorescence</topic><topic>Ion channels</topic><topic>Lasers</topic><topic>Localization</topic><topic>Methods</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondrial Dynamics</topic><topic>Morphology</topic><topic>Penicillin</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Voltage-Dependent Anion Channel 1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilic, Johannes</creatorcontrib><creatorcontrib>Oflaz, Furkan E</creatorcontrib><creatorcontrib>Gottschalk, Benjamin</creatorcontrib><creatorcontrib>Erdogan, Yusuf C</creatorcontrib><creatorcontrib>Graier, Wolfgang F</creatorcontrib><creatorcontrib>Malli, Roland</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilic, Johannes</au><au>Oflaz, Furkan E</au><au>Gottschalk, Benjamin</au><au>Erdogan, Yusuf C</au><au>Graier, Wolfgang F</au><au>Malli, Roland</au><au>Kahle, Philipp J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualizing VDAC1 in live cells using a tetracysteine tag</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-10-18</date><risdate>2024</risdate><volume>19</volume><issue>10</issue><spage>e0311107</spage><pages>e0311107-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 in living cells. To permit precise VDAC1 imaging, we utilized the tetracysteine (TC)-tag and visualized VDAC1 dynamics in living cells. TC-tagged VDAC1 had a cluster-like distribution on mitochondria. The labeling of TC-tagged VDAC1 was validated with immunofluorescence. The majority of VDAC1-TC-clusters were localized at endoplasmic reticulum (ER)-mitochondria contact sites. Notably, VDAC1 colocalized with BCL-2 Antagonist/Killer (BAK)-clusters upon apoptotic stimulation. Using this new tool, we were able to observe VDAC1-TC at mitochondrial fission sites. These findings highlight the suitability of the TC-tag for live-cell imaging of VDAC1, shedding light on the roles of VDAC1 in cellular processes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39423216</pmid><doi>10.1371/journal.pone.0311107</doi><tpages>e0311107</tpages><orcidid>https://orcid.org/0000-0001-6327-8729</orcidid><orcidid>https://orcid.org/0000-0003-1871-3298</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2024-10, Vol.19 (10), p.e0311107 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_3118206928 |
source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central Free |
subjects | Antibodies Apoptosis Bcl-2 protein Cell culture Cells Cells (biology) Clusters Cysteine Cysteine - metabolism Endoplasmic reticulum Endoplasmic Reticulum - metabolism Energy balance Glucose HeLa Cells Homeostasis Humans Imaging systems Immunofluorescence Ion channels Lasers Localization Methods Mitochondria Mitochondria - metabolism Mitochondrial Dynamics Morphology Penicillin Physiological aspects Proteins Voltage-Dependent Anion Channel 1 - metabolism |
title | Visualizing VDAC1 in live cells using a tetracysteine tag |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A28%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Visualizing%20VDAC1%20in%20live%20cells%20using%20a%20tetracysteine%20tag&rft.jtitle=PloS%20one&rft.au=Pilic,%20Johannes&rft.date=2024-10-18&rft.volume=19&rft.issue=10&rft.spage=e0311107&rft.pages=e0311107-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0311107&rft_dat=%3Cgale_plos_%3EA812727946%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c450t-1ca9d92f0e6e12b355a80036306c69762d3ba10f474dc78137bbe615f0e4f9e73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3118206928&rft_id=info:pmid/39423216&rft_galeid=A812727946&rfr_iscdi=true |