The SWI/SNF subunit ARID1B is important for regenerative ability of hematopoietic stem cells in normal hematopoiesis

The Switch/Sugar non-fermenting (SWI/SNF) nucleosome remodeling complexes are essential for normal hematopoiesis. The Brg1/Brm associated factor (BAF) is a form of mammalian SWI/SNF that is distinguished by the presence of either ARID1A or ARID1B protein. In this study, we used hematopoietic specifi...

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Bibliographic Details
Published in:PloS one 2024-10, Vol.19 (10), p.e0312616
Main Authors: Arnold, Olivia, Bluemn, Theresa, Stelloh, Cary, Rao, Sridhar, Zhu, Nan
Format: Article
Language:English
Subjects:
Animal models
Animals
Biology and Life Sciences
Blood tests
Bone marrow
BRG1 protein
Competition
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Engineering and Technology
Flow cytometry
Hematopoiesis
Hematopoietic stem cells
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Hematopoietic system
Hemoglobin
Hemopoiesis
Medical research
Medicine and Health Sciences
Medicine, Experimental
Mice
Mice, Knockout
Nucleoproteins
Physiological aspects
Progenitor cells
Proteins
Reagents
Regeneration
Research and Analysis Methods
Spleen
Stem cells
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:The Switch/Sugar non-fermenting (SWI/SNF) nucleosome remodeling complexes are essential for normal hematopoiesis. The Brg1/Brm associated factor (BAF) is a form of mammalian SWI/SNF that is distinguished by the presence of either ARID1A or ARID1B protein. In this study, we used hematopoietic specific Cre mouse models to assess the function of Arid1b in blood development. We found Arid1b loss did not affect steady state hematopoiesis or hematopoietic regeneration. Nonetheless, Arid1b null hematopoietic stem and progenitor cells have reduced ability to reconstitute hematopoietic system compared to wild type cells. Overall, our data indicate Arid1b is largely dispensable for normal hematopoiesis but impairs the regenerative ability of HSPCs.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0312616