Seasonal changes in activity of hypothalamic thyroid hormone system in different winter phenotypes of Djungarian hamster (Phodopus sungorus)

• Published in: PloS one 2024-10, Vol.19 (10), p.e0309591
• Main Authors: Przybylska-Piech, Anna S, Diedrich, Victoria, Herwig, Annika
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Animals; Body mass; Cricetinae; Female; Females; Gene expression; Gene polymorphism; Genotype & phenotype; Gonads; Hamsters; Hibernation; Hormones; Hypothalamic hormones; Hypothalamus; Hypothalamus - metabolism; Iodide peroxidase; Iodide Peroxidase - genetics; Iodide Peroxidase - metabolism; Iodothyronine Deiodinase Type II; Male; Metabolism; Neuroendocrine system; Phenotype; Phenotypes; Phodopus; Phodopus sungorus; Photoperiod; Photoperiods; Physiological aspects; Polymerase chain reaction; Polymorphism; Population studies; Seasonal variations; Seasons; Somatostatin; Temperature; Thyroid; Thyroid gland; Thyroid hormones; Thyroid Hormones - metabolism; Thyroid-stimulating hormone; Thyrotropin-releasing hormone; Thyrotropin-Releasing Hormone - genetics; Thyrotropin-Releasing Hormone - metabolism; Thyroxine deiodinase; Torpor; Ventricles (cerebral); Winter; Zoological research
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0309591

Although the Djungarian hamster (Phodopus sungorus) is a seasonality model, it presents substantial variability in winter acclimation. In response to short photoperiod, some individuals express a suite of winter traits such as low body mass, regressed gonads, white fur, and daily torpor, while others develop only some adjustments or maintain a summer phenotype. Despite comprehensive research, the mechanisms underlying polymorphism of winter phenotype are still unknown. We compared key elements of the hypothalamic thyroid hormone system, as well as the tanycyte architecture in hamsters of both sexes. Individuals presented different responses to short photoperiod characterized either as phenotypes (non-responder, partial-responder and full-responder) or photoresponsive index. We measured the expression of genes coding iodothyronine deiodinase 2 and 3, monocarboxylate transporter 8, thyrotropin-releasing hormone, and somatostatin in 40 individuals and counted the number of immunolabeled tanycyte processes in standardized regions of interest around the third ventricle in 30 individuals. Animals acclimated to short photoperiod presented a downregulation of diodinase 2 and somatostatin and an upregulation of deiodinase 3, as well as a decreased number of tanycyte processes, compared to long photoperiod-exposed individuals. Although phenotypes did not differ in gene expression, the higher the photoresponsive index, the lower was the deiodinase 2 expression and the higher the deiodinase 3 expression. Partial-responders and full-responders had less tanycyte processes than non-responders, and the number of tanycyte processes correlated with the photoresponsive index. Sexes differed neither in their seasonal response, nor hypothalamic gene expression, but females had more tanycyte processes. Our results are in accordance with studies emphasizing the pivotal role of thyroid hormones in seasonal response. We suggest that the whole spectrum of winter phenotypes exists within the population of Djungarian hamsters and that it is reflected also at the level of neuroendocrine regulation. However, the neuroendocrine underpinnings of winter phenotype polymorphism require further investigation.