Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line

Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2024-10, Vol.19 (10), p.e0311283
Main Authors: Collins, Catherine, Chaumont, Lise, Peruzzi, Mathilde, Jamak, Nedim, Boudinot, Pierre, Béjar, Julia, Moreno, Patricia, Álvarez Torres, Daniel, Collet, Bertrand
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological response modifiers
Biology and Life Sciences
Carp
Cell Line
Cells
Chinook salmon
Cloning
CRISPR
CRISPR-Cas Systems
DNA helicase
Double-stranded RNA
Drug resistance
Enzymes
Fish diseases
Fish Diseases - genetics
Fish Diseases - immunology
Fish Diseases - virology
Fish Proteins - genetics
Fish Proteins - metabolism
Fishes
Genes
Genetic aspects
Genome editing
Genomes
Genomics
Health aspects
Infection
Infections
Infectious pancreatic necrosis
Interferon
Interferon Type I - metabolism
Interferon-Induced Helicase, IFIH1 - genetics
Interferon-Induced Helicase, IFIH1 - metabolism
Life Sciences
Maximum likelihood method
Melanoma
Necrosis
Oncorhynchus tshawytscha
Plant virus diseases
Plant viruses
Proteins
Redundancy
Research and Analysis Methods
RNA
Salmon
Salmon - virology
Trout
Viral infections
Viruses
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c540t-600d6ea68d72b2fbd8cfd153e61c0c289ea783f7999191a264af3ade7329ff1b3
container_end_page
container_issue 10
container_start_page e0311283
container_title PloS one
container_volume 19
creator Collins, Catherine
Chaumont, Lise
Peruzzi, Mathilde
Jamak, Nedim
Boudinot, Pierre
Béjar, Julia
Moreno, Patricia
Álvarez Torres, Daniel
Collet, Bertrand
description Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs encoded by the ifih1 gene, binds to long dsRNA molecules during a viral infection and initiates production of type I interferon (IFN1) which orchestrates the antiviral response. In order to understand the contribution of MDA5 to viral resistance in fish cells, we have isolated a clonal Chinook salmon Oncorhynchus tshawytscha epithelial-like cell line invalidated for the ifih1 gene by CRISPR/Cas9 genome editing. We demonstrated that IFN1 induction is impaired in this cell line after infection with the Snakehead Rhabdovirus (SHRV), the Salmon Alphavirus (SAV) or Nervous Necrosis Virus (NNV). The cell line, however, did not show any increase in cytopathic effect when infected with SHRV or SAV. Similarly, no cytopathic effect was observed in the ifih1-/- cell line when infected with Infectious Pancreatic Necrosis Virus (IPNV), Infectious Haemorrhagic Necrotic Virus (IHNV). These results indicate the redundancy of the antiviral innate defence system in CHSE-derived cells, which helps with circumventing viral evasion strategies.
doi_str_mv 10.1371/journal.pone.0311283
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3123776376</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A812225531</galeid><sourcerecordid>A812225531</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-600d6ea68d72b2fbd8cfd153e61c0c289ea783f7999191a264af3ade7329ff1b3</originalsourceid><addsrcrecordid>eNqNk21r2zAQx83YWLts32BsgsFYXyTVgy3br0YI3VoIBPb0VijyKVZnS5klZ8s-_eTWLUnpi2GBxen3vzud7pLkNcEzwnJyfu36zspmtnUWZpgRQgv2JDklJaNTTjF7erA_SV54f41xxgrOnycnrEwxoYydJn8vtAYVkNNIosZ5P-xCDaitZHZutKkJ2oAF5OyNWdpgdqaTDerAGx-kVYCMjeJFbaxzP5GXTRvhlVWuq_dW1b1Hwdfy9z54VUukoGlQYyy8TJ5p2Xh4Nf4nyfdPF98Wl9Pl6vPVYr6cqizFYcoxrjhIXlQ5XVO9rgqlK5Ix4ERhRYsSZF4wnZdlSUoiKU-lZrKCnNFSa7Jmk-Ttrd9tvJ8Yy-YFiwXIc87imiQfR6Jft1ApsCHeUGw708puL5w04vjEmlps3E4QkuY0ho0ezm491A90l_OlGGw4LSjNU7Ijkf0wRuvcrx58EK3xQ1WkBdcPiRHO84zfJPbuAfp4-iO1kQ0IY7WLSarBqZgXhFKaZWwIO3uEil8FrVGxibSJ9iPB2ZEgMgH-hI3svRdXX7_8P7v6ccy-P2BrkE2ovWv6YJz1x2B6C6ouNmYH-r6yBIthBu6qIYYZEOMMRNmbw9e8F901PfsH92QAvA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3123776376</pqid></control><display><type>article</type><title>Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database</source><source>Coronavirus Research Database</source><creator>Collins, Catherine ; Chaumont, Lise ; Peruzzi, Mathilde ; Jamak, Nedim ; Boudinot, Pierre ; Béjar, Julia ; Moreno, Patricia ; Álvarez Torres, Daniel ; Collet, Bertrand</creator><contributor>Ortega-Villaizan, Maria del Mar</contributor><creatorcontrib>Collins, Catherine ; Chaumont, Lise ; Peruzzi, Mathilde ; Jamak, Nedim ; Boudinot, Pierre ; Béjar, Julia ; Moreno, Patricia ; Álvarez Torres, Daniel ; Collet, Bertrand ; Ortega-Villaizan, Maria del Mar</creatorcontrib><description>Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs encoded by the ifih1 gene, binds to long dsRNA molecules during a viral infection and initiates production of type I interferon (IFN1) which orchestrates the antiviral response. In order to understand the contribution of MDA5 to viral resistance in fish cells, we have isolated a clonal Chinook salmon Oncorhynchus tshawytscha epithelial-like cell line invalidated for the ifih1 gene by CRISPR/Cas9 genome editing. We demonstrated that IFN1 induction is impaired in this cell line after infection with the Snakehead Rhabdovirus (SHRV), the Salmon Alphavirus (SAV) or Nervous Necrosis Virus (NNV). The cell line, however, did not show any increase in cytopathic effect when infected with SHRV or SAV. Similarly, no cytopathic effect was observed in the ifih1-/- cell line when infected with Infectious Pancreatic Necrosis Virus (IPNV), Infectious Haemorrhagic Necrotic Virus (IHNV). These results indicate the redundancy of the antiviral innate defence system in CHSE-derived cells, which helps with circumventing viral evasion strategies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0311283</identifier><identifier>PMID: 39401233</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biological response modifiers ; Biology and Life Sciences ; Carp ; Cell Line ; Cells ; Chinook salmon ; Cloning ; CRISPR ; CRISPR-Cas Systems ; DNA helicase ; Double-stranded RNA ; Drug resistance ; Enzymes ; Fish diseases ; Fish Diseases - genetics ; Fish Diseases - immunology ; Fish Diseases - virology ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Fishes ; Genes ; Genetic aspects ; Genome editing ; Genomes ; Genomics ; Health aspects ; Infection ; Infections ; Infectious pancreatic necrosis ; Interferon ; Interferon Type I - metabolism ; Interferon-Induced Helicase, IFIH1 - genetics ; Interferon-Induced Helicase, IFIH1 - metabolism ; Life Sciences ; Maximum likelihood method ; Melanoma ; Necrosis ; Oncorhynchus tshawytscha ; Plant virus diseases ; Plant viruses ; Proteins ; Redundancy ; Research and Analysis Methods ; RNA ; Salmon ; Salmon - virology ; Trout ; Viral infections ; Viruses</subject><ispartof>PloS one, 2024-10, Vol.19 (10), p.e0311283</ispartof><rights>Copyright: © 2024 Collins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Collins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><rights>2024 Collins et al 2024 Collins et al</rights><rights>2024 Collins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c540t-600d6ea68d72b2fbd8cfd153e61c0c289ea783f7999191a264af3ade7329ff1b3</cites><orcidid>0000-0003-2795-2653</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3123776376/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3123776376?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,38516,43895,44590,53791,53793,74412,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39401233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04822741$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Ortega-Villaizan, Maria del Mar</contributor><creatorcontrib>Collins, Catherine</creatorcontrib><creatorcontrib>Chaumont, Lise</creatorcontrib><creatorcontrib>Peruzzi, Mathilde</creatorcontrib><creatorcontrib>Jamak, Nedim</creatorcontrib><creatorcontrib>Boudinot, Pierre</creatorcontrib><creatorcontrib>Béjar, Julia</creatorcontrib><creatorcontrib>Moreno, Patricia</creatorcontrib><creatorcontrib>Álvarez Torres, Daniel</creatorcontrib><creatorcontrib>Collet, Bertrand</creatorcontrib><title>Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs encoded by the ifih1 gene, binds to long dsRNA molecules during a viral infection and initiates production of type I interferon (IFN1) which orchestrates the antiviral response. In order to understand the contribution of MDA5 to viral resistance in fish cells, we have isolated a clonal Chinook salmon Oncorhynchus tshawytscha epithelial-like cell line invalidated for the ifih1 gene by CRISPR/Cas9 genome editing. We demonstrated that IFN1 induction is impaired in this cell line after infection with the Snakehead Rhabdovirus (SHRV), the Salmon Alphavirus (SAV) or Nervous Necrosis Virus (NNV). The cell line, however, did not show any increase in cytopathic effect when infected with SHRV or SAV. Similarly, no cytopathic effect was observed in the ifih1-/- cell line when infected with Infectious Pancreatic Necrosis Virus (IPNV), Infectious Haemorrhagic Necrotic Virus (IHNV). These results indicate the redundancy of the antiviral innate defence system in CHSE-derived cells, which helps with circumventing viral evasion strategies.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biological response modifiers</subject><subject>Biology and Life Sciences</subject><subject>Carp</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Chinook salmon</subject><subject>Cloning</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems</subject><subject>DNA helicase</subject><subject>Double-stranded RNA</subject><subject>Drug resistance</subject><subject>Enzymes</subject><subject>Fish diseases</subject><subject>Fish Diseases - genetics</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - virology</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Fishes</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genome editing</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Infection</subject><subject>Infections</subject><subject>Infectious pancreatic necrosis</subject><subject>Interferon</subject><subject>Interferon Type I - metabolism</subject><subject>Interferon-Induced Helicase, IFIH1 - genetics</subject><subject>Interferon-Induced Helicase, IFIH1 - metabolism</subject><subject>Life Sciences</subject><subject>Maximum likelihood method</subject><subject>Melanoma</subject><subject>Necrosis</subject><subject>Oncorhynchus tshawytscha</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>Proteins</subject><subject>Redundancy</subject><subject>Research and Analysis Methods</subject><subject>RNA</subject><subject>Salmon</subject><subject>Salmon - virology</subject><subject>Trout</subject><subject>Viral infections</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>COVID</sourceid><sourceid>PIMPY</sourceid><recordid>eNqNk21r2zAQx83YWLts32BsgsFYXyTVgy3br0YI3VoIBPb0VijyKVZnS5klZ8s-_eTWLUnpi2GBxen3vzud7pLkNcEzwnJyfu36zspmtnUWZpgRQgv2JDklJaNTTjF7erA_SV54f41xxgrOnycnrEwxoYydJn8vtAYVkNNIosZ5P-xCDaitZHZutKkJ2oAF5OyNWdpgdqaTDerAGx-kVYCMjeJFbaxzP5GXTRvhlVWuq_dW1b1Hwdfy9z54VUukoGlQYyy8TJ5p2Xh4Nf4nyfdPF98Wl9Pl6vPVYr6cqizFYcoxrjhIXlQ5XVO9rgqlK5Ix4ERhRYsSZF4wnZdlSUoiKU-lZrKCnNFSa7Jmk-Ttrd9tvJ8Yy-YFiwXIc87imiQfR6Jft1ApsCHeUGw708puL5w04vjEmlps3E4QkuY0ho0ezm491A90l_OlGGw4LSjNU7Ijkf0wRuvcrx58EK3xQ1WkBdcPiRHO84zfJPbuAfp4-iO1kQ0IY7WLSarBqZgXhFKaZWwIO3uEil8FrVGxibSJ9iPB2ZEgMgH-hI3svRdXX7_8P7v6ccy-P2BrkE2ovWv6YJz1x2B6C6ouNmYH-r6yBIthBu6qIYYZEOMMRNmbw9e8F901PfsH92QAvA</recordid><startdate>20241014</startdate><enddate>20241014</enddate><creator>Collins, Catherine</creator><creator>Chaumont, Lise</creator><creator>Peruzzi, Mathilde</creator><creator>Jamak, Nedim</creator><creator>Boudinot, Pierre</creator><creator>Béjar, Julia</creator><creator>Moreno, Patricia</creator><creator>Álvarez Torres, Daniel</creator><creator>Collet, Bertrand</creator><general>Public Library of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2795-2653</orcidid></search><sort><creationdate>20241014</creationdate><title>Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line</title><author>Collins, Catherine ; Chaumont, Lise ; Peruzzi, Mathilde ; Jamak, Nedim ; Boudinot, Pierre ; Béjar, Julia ; Moreno, Patricia ; Álvarez Torres, Daniel ; Collet, Bertrand</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-600d6ea68d72b2fbd8cfd153e61c0c289ea783f7999191a264af3ade7329ff1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biological response modifiers</topic><topic>Biology and Life Sciences</topic><topic>Carp</topic><topic>Cell Line</topic><topic>Cells</topic><topic>Chinook salmon</topic><topic>Cloning</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems</topic><topic>DNA helicase</topic><topic>Double-stranded RNA</topic><topic>Drug resistance</topic><topic>Enzymes</topic><topic>Fish diseases</topic><topic>Fish Diseases - genetics</topic><topic>Fish Diseases - immunology</topic><topic>Fish Diseases - virology</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Fishes</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genome editing</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Infection</topic><topic>Infections</topic><topic>Infectious pancreatic necrosis</topic><topic>Interferon</topic><topic>Interferon Type I - metabolism</topic><topic>Interferon-Induced Helicase, IFIH1 - genetics</topic><topic>Interferon-Induced Helicase, IFIH1 - metabolism</topic><topic>Life Sciences</topic><topic>Maximum likelihood method</topic><topic>Melanoma</topic><topic>Necrosis</topic><topic>Oncorhynchus tshawytscha</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>Proteins</topic><topic>Redundancy</topic><topic>Research and Analysis Methods</topic><topic>RNA</topic><topic>Salmon</topic><topic>Salmon - virology</topic><topic>Trout</topic><topic>Viral infections</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collins, Catherine</creatorcontrib><creatorcontrib>Chaumont, Lise</creatorcontrib><creatorcontrib>Peruzzi, Mathilde</creatorcontrib><creatorcontrib>Jamak, Nedim</creatorcontrib><creatorcontrib>Boudinot, Pierre</creatorcontrib><creatorcontrib>Béjar, Julia</creatorcontrib><creatorcontrib>Moreno, Patricia</creatorcontrib><creatorcontrib>Álvarez Torres, Daniel</creatorcontrib><creatorcontrib>Collet, Bertrand</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest - Health &amp; Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collins, Catherine</au><au>Chaumont, Lise</au><au>Peruzzi, Mathilde</au><au>Jamak, Nedim</au><au>Boudinot, Pierre</au><au>Béjar, Julia</au><au>Moreno, Patricia</au><au>Álvarez Torres, Daniel</au><au>Collet, Bertrand</au><au>Ortega-Villaizan, Maria del Mar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-10-14</date><risdate>2024</risdate><volume>19</volume><issue>10</issue><spage>e0311283</spage><pages>e0311283-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs encoded by the ifih1 gene, binds to long dsRNA molecules during a viral infection and initiates production of type I interferon (IFN1) which orchestrates the antiviral response. In order to understand the contribution of MDA5 to viral resistance in fish cells, we have isolated a clonal Chinook salmon Oncorhynchus tshawytscha epithelial-like cell line invalidated for the ifih1 gene by CRISPR/Cas9 genome editing. We demonstrated that IFN1 induction is impaired in this cell line after infection with the Snakehead Rhabdovirus (SHRV), the Salmon Alphavirus (SAV) or Nervous Necrosis Virus (NNV). The cell line, however, did not show any increase in cytopathic effect when infected with SHRV or SAV. Similarly, no cytopathic effect was observed in the ifih1-/- cell line when infected with Infectious Pancreatic Necrosis Virus (IPNV), Infectious Haemorrhagic Necrotic Virus (IHNV). These results indicate the redundancy of the antiviral innate defence system in CHSE-derived cells, which helps with circumventing viral evasion strategies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39401233</pmid><doi>10.1371/journal.pone.0311283</doi><tpages>e0311283</tpages><orcidid>https://orcid.org/0000-0003-2795-2653</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2024-10, Vol.19 (10), p.e0311283
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_3123776376
source Open Access: PubMed Central; Publicly Available Content Database; Coronavirus Research Database
subjects Analysis
Animals
Biological response modifiers
Biology and Life Sciences
Carp
Cell Line
Cells
Chinook salmon
Cloning
CRISPR
CRISPR-Cas Systems
DNA helicase
Double-stranded RNA
Drug resistance
Enzymes
Fish diseases
Fish Diseases - genetics
Fish Diseases - immunology
Fish Diseases - virology
Fish Proteins - genetics
Fish Proteins - metabolism
Fishes
Genes
Genetic aspects
Genome editing
Genomes
Genomics
Health aspects
Infection
Infections
Infectious pancreatic necrosis
Interferon
Interferon Type I - metabolism
Interferon-Induced Helicase, IFIH1 - genetics
Interferon-Induced Helicase, IFIH1 - metabolism
Life Sciences
Maximum likelihood method
Melanoma
Necrosis
Oncorhynchus tshawytscha
Plant virus diseases
Plant viruses
Proteins
Redundancy
Research and Analysis Methods
RNA
Salmon
Salmon - virology
Trout
Viral infections
Viruses
title Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A23%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20a%20loss%20of%20the%20mda5/ifih1%20gene%20on%20the%20antiviral%20resistance%20in%20a%20Chinook%20salmon%20Oncorhynchus%20tshawytscha%20cell%20line&rft.jtitle=PloS%20one&rft.au=Collins,%20Catherine&rft.date=2024-10-14&rft.volume=19&rft.issue=10&rft.spage=e0311283&rft.pages=e0311283-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0311283&rft_dat=%3Cgale_plos_%3EA812225531%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c540t-600d6ea68d72b2fbd8cfd153e61c0c289ea783f7999191a264af3ade7329ff1b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3123776376&rft_id=info:pmid/39401233&rft_galeid=A812225531&rfr_iscdi=true