Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity

• Published in: PloS one 2024-11, Vol.19 (11), p.e0313548
• Main Authors: Ghosh, Supriyo, Ghosh, Amlan Jyoti, Islam, Rejuan, Sarkar, Sagar, Saha, Tilak
• Format: Article
• Language: English
• Subjects: Albinism; Analysis; Animal experimentation; Animals; Bacteria; Biology and Life Sciences; Body fat; Body weight; Body weight gain; Care and treatment; Cholesterol; Committees; Complications and side effects; Diagnosis; Diet; Diet, High-Fat - adverse effects; Dietary intake; Dietary supplements; Ethylenediaminetetraacetic acid; Evaluation; Feces; Fermented food; Food intake; Gastrointestinal Microbiome - drug effects; Glucose; Health aspects; High fat diet; Hyperglycemia; Inflammation; Insulin resistance; Intestinal microflora; Lactic acid; Lactic acid bacteria; Lactobacilli; Lactobacillus plantarum; Lipoproteins; Liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Medicine and Health Sciences; Metabolic disorders; Metagenomics; Mice; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Obesity; Oral administration; Oxidative stress; Oxidative Stress - drug effects; Parameters; Probiotics; Probiotics - administration & dosage; Probiotics - pharmacology; Sequences; Strain; Traditional foods; Triglycerides
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0313548

Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p