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Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity
Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacil...
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| Published in: | PloS one 2024-11, Vol.19 (11), p.e0313548 |
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| creator | Ghosh, Supriyo Ghosh, Amlan Jyoti Islam, Rejuan Sarkar, Sagar Saha, Tilak |
| description | Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p |
| doi_str_mv | 10.1371/journal.pone.0313548 |
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Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p<0.05) restore metabolic health by normalizing glycemic, lipidomic, hepatic parameters, oxidative stress and inflammatory parameters. Moreover, LP group (7.08±0.52 mg/g) showed significantly (p<0.001) decreased hepatic triglyceride level compared to HFD group (20.07±1.32 mg/g). L. plantarum KAD improved the adipocytic, and colonic histomorphology with significantly better scoring pattern. LP group (1.83±0.41) showed a significantly (p<0.001) reduced hepatic score compared to negative control group (5.00±0.63), showing reduced hepatosteatosis, and immune infiltration. The strain modulated gut health by altering its microbial composition positively towards normalization. In conclusion, the results of the experiment suggest that prophylactic L. plantarum KAD administration has beneficial effects on the onset of HFD induced hepatic complications in mice. Further studies are needed, on this strain for its clinical use as dietary supplement.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0313548</identifier><identifier>PMID: 39531444</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Albinism ; Analysis ; Animal experimentation ; Animals ; Bacteria ; Biology and Life Sciences ; Body fat ; Body weight ; Body weight gain ; Care and treatment ; Cholesterol ; Committees ; Complications and side effects ; Diagnosis ; Diet ; Diet, High-Fat - adverse effects ; Dietary intake ; Dietary supplements ; Ethylenediaminetetraacetic acid ; Evaluation ; Feces ; Fermented food ; Food intake ; Gastrointestinal Microbiome - drug effects ; Glucose ; Health aspects ; High fat diet ; Hyperglycemia ; Inflammation ; Insulin resistance ; Intestinal microflora ; Lactic acid ; Lactic acid bacteria ; Lactobacilli ; Lactobacillus plantarum ; Lipoproteins ; Liver ; Liver - drug effects ; Liver - metabolism ; Liver - pathology ; Male ; Medicine and Health Sciences ; Metabolic disorders ; Metagenomics ; Mice ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Obesity ; Oral administration ; Oxidative stress ; Oxidative Stress - drug effects ; Parameters ; Probiotics ; Probiotics - administration & dosage ; Probiotics - pharmacology ; Sequences ; Strain ; Traditional foods ; Triglycerides</subject><ispartof>PloS one, 2024-11, Vol.19 (11), p.e0313548</ispartof><rights>Copyright: © 2024 Ghosh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Ghosh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Ghosh et al 2024 Ghosh et al</rights><rights>2024 Ghosh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c526t-fbba4d9fd7467286f351d1fddd4150d22fe0b43857f8373913a2f7898670863</cites><orcidid>0000-0001-8287-901X ; 0000-0001-6960-3596</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3127539233/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3127539233?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,25734,27905,27906,36993,36994,44571,53772,53774,74875</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39531444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rashwan, Ahmed Khalafallah Sadeq</contributor><creatorcontrib>Ghosh, Supriyo</creatorcontrib><creatorcontrib>Ghosh, Amlan Jyoti</creatorcontrib><creatorcontrib>Islam, Rejuan</creatorcontrib><creatorcontrib>Sarkar, Sagar</creatorcontrib><creatorcontrib>Saha, Tilak</creatorcontrib><title>Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p<0.05) restore metabolic health by normalizing glycemic, lipidomic, hepatic parameters, oxidative stress and inflammatory parameters. Moreover, LP group (7.08±0.52 mg/g) showed significantly (p<0.001) decreased hepatic triglyceride level compared to HFD group (20.07±1.32 mg/g). L. plantarum KAD improved the adipocytic, and colonic histomorphology with significantly better scoring pattern. LP group (1.83±0.41) showed a significantly (p<0.001) reduced hepatic score compared to negative control group (5.00±0.63), showing reduced hepatosteatosis, and immune infiltration. The strain modulated gut health by altering its microbial composition positively towards normalization. In conclusion, the results of the experiment suggest that prophylactic L. plantarum KAD administration has beneficial effects on the onset of HFD induced hepatic complications in mice. Further studies are needed, on this strain for its clinical use as dietary supplement.</description><subject>Albinism</subject><subject>Analysis</subject><subject>Animal experimentation</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Body fat</subject><subject>Body weight</subject><subject>Body weight gain</subject><subject>Care and treatment</subject><subject>Cholesterol</subject><subject>Committees</subject><subject>Complications and side effects</subject><subject>Diagnosis</subject><subject>Diet</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Dietary intake</subject><subject>Dietary supplements</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Evaluation</subject><subject>Feces</subject><subject>Fermented food</subject><subject>Food intake</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Glucose</subject><subject>Health aspects</subject><subject>High fat diet</subject><subject>Hyperglycemia</subject><subject>Inflammation</subject><subject>Insulin resistance</subject><subject>Intestinal microflora</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactobacilli</subject><subject>Lactobacillus plantarum</subject><subject>Lipoproteins</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic disorders</subject><subject>Metagenomics</subject><subject>Mice</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Obesity</subject><subject>Oral administration</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Parameters</subject><subject>Probiotics</subject><subject>Probiotics - administration & dosage</subject><subject>Probiotics - pharmacology</subject><subject>Sequences</subject><subject>Strain</subject><subject>Traditional foods</subject><subject>Triglycerides</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggsISG42CU-J9ygqpxWVKrUIm4tx4esKydOYwfog_C-eHfTqot6gXxha_zNP54ZT1E8h-USYg7fXYZp7KVfDqE3yxJDTEn1oDiENUYLhkr88M75oHgS42VZUlwx9rg4wDXFkBByWPw5lSqFRirn_RTB4GWf5Dh14NvxRzCMIRmVIpCtdH1MYO3a9cLKBLQzaeF6PSmjwdoMMjkFVOgG71Q-hz4C14OLXy5mZ9-4PoDOKfMenAdvQLD51nrZdVsWyF6DdkrZmEw7unT9tHhkpY_m2bwfFRefP30_-bo4PfuyOjk-XSiKWFrYppFE11ZzwjiqmMUUami11gTSUiNkTdkQXFFuK8xxDbFElld1xXhZMXxUvNypDj5EMdczCgwRp7hGGGditSN0kJdiGF0nx2sRpBNbQxhbIcecujcC8SxtFVFKU8Ir3jSm0UoiXteIUdZkrQ9ztKnpjFamT6P0e6L7N71bizb8FBBSyljFs8KbWWEMV5OJSXQuKuNzz0yYtg-vOIOwJhl99Q96f3oz1cqcQW5JyIHVRlQcV5ByUiK6Cbu8h8pLm9zT_Pusy_Y9h7d7DplJ5ndq5RSjWF2c_z979mOffX2HXRvp0zoGP23_2z5IdqAaQ4yjsbdVhqXYDM9NNcRmeMQ8PNntxd0O3TrdTAv-CzyLFmA</recordid><startdate>20241112</startdate><enddate>20241112</enddate><creator>Ghosh, Supriyo</creator><creator>Ghosh, Amlan Jyoti</creator><creator>Islam, Rejuan</creator><creator>Sarkar, Sagar</creator><creator>Saha, Tilak</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8287-901X</orcidid><orcidid>https://orcid.org/0000-0001-6960-3596</orcidid></search><sort><creationdate>20241112</creationdate><title>Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity</title><author>Ghosh, Supriyo ; Ghosh, Amlan Jyoti ; Islam, Rejuan ; Sarkar, Sagar ; Saha, Tilak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-fbba4d9fd7467286f351d1fddd4150d22fe0b43857f8373913a2f7898670863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Albinism</topic><topic>Analysis</topic><topic>Animal experimentation</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biology and Life Sciences</topic><topic>Body fat</topic><topic>Body weight</topic><topic>Body weight gain</topic><topic>Care and treatment</topic><topic>Cholesterol</topic><topic>Committees</topic><topic>Complications and side effects</topic><topic>Diagnosis</topic><topic>Diet</topic><topic>Diet, High-Fat - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghosh, Supriyo</au><au>Ghosh, Amlan Jyoti</au><au>Islam, Rejuan</au><au>Sarkar, Sagar</au><au>Saha, Tilak</au><au>Rashwan, Ahmed Khalafallah Sadeq</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-11-12</date><risdate>2024</risdate><volume>19</volume><issue>11</issue><spage>e0313548</spage><pages>e0313548-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed the beneficial effects of probiotics in HFD fed mice with hepatic complications. Some probiotic Lactic acid bacteria (LAB) e.g. Lactobacillus plantarum have drawn our attention in managing hepatic complications. Here, we aim to elucidate the protective effects of L. plantarum KAD strain, isolated from ethnic fermented food 'Kinema' in HFD-fed mice as, a preventive approach. Eighteen Swiss albino mice were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and HFD-fed with oral L. plantarum KAD supplementation (LP). All the experimental groups were subjected to specific diet according to grouping for eight weeks. After completion of the regime, subjects were anesthetized and sacrificed. Organs, blood, and fecal samples were collected and stored appropriately. Physical indices, including body weight gain, organ co-efficients were calculated along with assessment of glycemic, lipidomic, hepatic, oxidative stress, inflammatory, and histological parameters. Gut microbiota analysis was performed using 16s V3-V4 fecal metagenomic profiling, and sequencing were done using Illumina Miseq system. Oral administration of L. plantarum KAD is found to significantly (p<0.05) restore metabolic health by normalizing glycemic, lipidomic, hepatic parameters, oxidative stress and inflammatory parameters. Moreover, LP group (7.08±0.52 mg/g) showed significantly (p<0.001) decreased hepatic triglyceride level compared to HFD group (20.07±1.32 mg/g). L. plantarum KAD improved the adipocytic, and colonic histomorphology with significantly better scoring pattern. LP group (1.83±0.41) showed a significantly (p<0.001) reduced hepatic score compared to negative control group (5.00±0.63), showing reduced hepatosteatosis, and immune infiltration. The strain modulated gut health by altering its microbial composition positively towards normalization. In conclusion, the results of the experiment suggest that prophylactic L. plantarum KAD administration has beneficial effects on the onset of HFD induced hepatic complications in mice. Further studies are needed, on this strain for its clinical use as dietary supplement.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39531444</pmid><doi>10.1371/journal.pone.0313548</doi><tpages>e0313548</tpages><orcidid>https://orcid.org/0000-0001-8287-901X</orcidid><orcidid>https://orcid.org/0000-0001-6960-3596</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-11, Vol.19 (11), p.e0313548 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3127539233 |
| source | Publicly Available Content Database; PubMed Central(OpenAccess) |
| subjects | Albinism Analysis Animal experimentation Animals Bacteria Biology and Life Sciences Body fat Body weight Body weight gain Care and treatment Cholesterol Committees Complications and side effects Diagnosis Diet Diet, High-Fat - adverse effects Dietary intake Dietary supplements Ethylenediaminetetraacetic acid Evaluation Feces Fermented food Food intake Gastrointestinal Microbiome - drug effects Glucose Health aspects High fat diet Hyperglycemia Inflammation Insulin resistance Intestinal microflora Lactic acid Lactic acid bacteria Lactobacilli Lactobacillus plantarum Lipoproteins Liver Liver - drug effects Liver - metabolism Liver - pathology Male Medicine and Health Sciences Metabolic disorders Metagenomics Mice Microbiota Microbiota (Symbiotic organisms) Microorganisms Obesity Oral administration Oxidative stress Oxidative Stress - drug effects Parameters Probiotics Probiotics - administration & dosage Probiotics - pharmacology Sequences Strain Traditional foods Triglycerides |
| title | Lactobacillus plantarum KAD protects against high-fat diet-induced hepatic complications in Swiss albino mice: Role of inflammation and gut integrity |
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