The effects of secretome of umbilical cord mesenchymal stem cells on regeneration of sciatic nerve defects in Sprague dawley rats

Current treatments for peripheral nerve defects are suboptimal. Mesenchymal stem cell (MSC) implantation holds promise, with studies indicating their efficacy through the secretome. This study aims to assess the secretome's potency in regenerating peripheral nerve defects. Twenty-eight spraque...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2024-12, Vol.19 (12), p.e0310467
Main Authors: Kurniawan, Aryadi, Dilogo, Ismail Hadisoebroto, Pawitan, Jeanne Adiwinata, Widodo, Wahyu, Oesman, Ihsan, Martinus, Ade
Format: Article
Language:English
Subjects:
Animals
Autografts
Axons
Biology and Life Sciences
Defects
Effectiveness
Gastrocnemius muscle
Growth factors
Histomorphometry
Humans
Implantation
Injuries
Male
Medicine and Health Sciences
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Muscle, Skeletal - metabolism
Muscles
Nerve Regeneration - physiology
Peripheral nerves
Rats
Rats, Sprague-Dawley
Recovery of function
Regeneration
Sciatic nerve
Sciatic Nerve - injuries
Sciatic Nerve - metabolism
Secretome
Secretome - metabolism
Silicones
Stem cells
Surgery
Transplantation of organs, tissues, etc
Umbilical cord
Umbilical Cord - cytology
Variance analysis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Current treatments for peripheral nerve defects are suboptimal. Mesenchymal stem cell (MSC) implantation holds promise, with studies indicating their efficacy through the secretome. This study aims to assess the secretome's potency in regenerating peripheral nerve defects. Twenty-eight spraque dawley (SD) rats were divided into four groups, with a 10mm segmental sciatic nerve defect created. Group I received autografts, while Groups II, III, and IV had proximal and distal nerve stumps fixed with a conduit. Group II received MSC implantation, Group III received umbilical cord MSC secretome, and Group IV was treated with silicone conduits. Motoric recovery was assessed using the Sciatic Functional Index (SFI) at 6, 9, and 12 weeks. After 12 weeks, rats were sacrificed for measurements of gastrocnemius muscle weight ratio and sciatic nerve histomorphometry. The highest ratio of the gastrocnemius muscle were found in groups 1 and 3, namely 0.7 ± 0.06 and 0.7 ± 0.2 (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0310467