Label-free determination of diffusion coefficients at the nanoscale through modelling of the Surface Plasmon Resonance signal

Surface plasmon resonance (SPR) is normally used to measure the kinetic parameters of biomolecular interactions between a molecule immobilized on a gold surface and another one flowing in a microfluidic channel above the surface. During the SPR measurements, convection-diffusion phenomena occur insi...

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Bibliographic Details
Published in:PloS one 2025-01, Vol.20 (1), p.e0312594
Main Authors: Zingale, Gabriele Antonio, Pandino, Irene, Calcagno, Damiano, Perina, Maria Luisa, Tuccitto, Nunzio, Grasso, Giuseppe
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Biomolecules
Convection
Diffusion
Diffusion coefficient
Engineering and Technology
Flow velocity
Gold - chemistry
Investigations
Kinetics
Lab Protocol
Ligands
Medicine and Health Sciences
Methods
Microfluidics
Models, Theoretical
Molecular interactions
Nanotechnology - methods
NMR
Nuclear magnetic resonance
Parameters
Physical Sciences
Protocol
Reproducibility
Resonance
Reynolds number
Surface plasmon resonance
Surface Plasmon Resonance - methods
Viscosity
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Summary:Surface plasmon resonance (SPR) is normally used to measure the kinetic parameters of biomolecular interactions between a molecule immobilized on a gold surface and another one flowing in a microfluidic channel above the surface. During the SPR measurements, convection-diffusion phenomena occur inside the microfluidic channels, but they are generally minimized by appropriate experimental setup in order to obtain diffusion free kinetic parameters of the molecular interactions. In this work, for the first time, a commercial SPR apparatus has been used to obtain non canonical scientific parameters. Indeed, a specifically designed SPR experimental setup is described for carrying out measurements of the diffusion coefficient (D) of molecules in solutions. The high precision and reproducibility of the approach, as well as the wide applicability of the newly proposed SPR based method for the measurement of D of many different molecules and biomolecules, are here demonstrated and illustrated in detail.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0312594