Measurement of unidirectional Pi to ATP flux in human visual cortex at 7 T by using in vivo31P magnetic resonance spectroscopy

Taking advantage of the high NMR detection sensitivity and the large chemical shift dispersion offered by ultra-high field strength of 7 T, the effect of magnetization transfer on inorganic phosphate (P i ) resonance during saturation of γ-ATP resonance, mediated by the ATP synthesis reaction, was o...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-11, Vol.100 (24), p.14409
Main Authors: Hao Lei, Kamil Ugurbil, Wei Chen
Format: Article
Language:English
Online Access:Get full text
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Summary:Taking advantage of the high NMR detection sensitivity and the large chemical shift dispersion offered by ultra-high field strength of 7 T, the effect of magnetization transfer on inorganic phosphate (P i ) resonance during saturation of γ-ATP resonance, mediated by the ATP synthesis reaction, was observed noninvasively in the human primary visual cortex by using in vivo 31 P magnetic resonance spectroscopy. The unidirectional flux from P i to ATP was measured by using progressive saturation transfer experiments. The cerebral ATP synthesis rate in the human primary visual cortex measured by 31 P magnetic resonance spectroscopy in this study was 12.1 ± 2.8 μmol ATP/g per min, which agreed well with the value that was calculated indirectly from the cerebral metabolic rate of glucose consumption reported previously.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2332656100