Direct Observation of Tiers in the Energy Landscape of a Chromoprotein: A Single-Molecule Study

Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigm...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-12, Vol.100 (26), p.15534-15538
Main Authors: Hofmann, Clemens, Aartsma, Thijs J., Michel, Hartmut, Köhler, Jürgen
Format: Article
Language:English
Subjects:
Absorption spectra
Amino acids
Atoms
Bacterial Proteins - chemistry
Biological Sciences
Biophysics
Chromophores
Energy
Laser spectroscopy
Lasers
Light-Harvesting Protein Complexes - chemistry
Line spectra
Molecules
Pigments
Proteins
Rhodospirillum - chemistry
Spectrometry, Fluorescence
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Summary:Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigment absorptions as a function of time. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. Additionally, a correlation between the average barrier height within a distinct tier and the time scale of the structural fluctuations is observed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2533896100