PDSM, a Motif for Phosphorylation-Dependent SUMO Modification

SUMO (small ubiquitin-like modifier) modification regulates many cellular processes, including transcription. Although sumoylation often occurs on specific lysines within the consensus tetrapeptide ΨKxE, other modifications, such as phosphorylation, may regulate the sumoylation of a substrate. We ha...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-01, Vol.103 (1), p.45-50
Main Authors: Hietakangas, Ville, Anckar, Julius, Blomster, Henri A., Fujimoto, Mitsuaki, Palvimo, Jorma J., Nakai, Akira, Sistonen, Lea
Format: Article
Language:English
Subjects:
Amino Acid Motifs - genetics
Antibodies
Biochemistry
Biological Sciences
Blotting, Western
Consensus Sequence - genetics
COS cells
Electrophoretic Mobility Shift Assay
Gene expression regulation
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Immunoprecipitation
Luciferases
Nuclear receptors
Phosphorylation
Plasmids
Proteins
Receptors
Regulator genes
Regulatory Elements, Transcriptional - genetics
Reverse Transcriptase Polymerase Chain Reaction
Small Ubiquitin-Related Modifier Proteins - metabolism
Substrates
Sumo
Western blotting
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Summary:SUMO (small ubiquitin-like modifier) modification regulates many cellular processes, including transcription. Although sumoylation often occurs on specific lysines within the consensus tetrapeptide ΨKxE, other modifications, such as phosphorylation, may regulate the sumoylation of a substrate. We have discovered PDSM (phosphorylation-dependent sumoylation motif), composed of a SUMO consensus site and an adjacent proline-directed phosphorylation site (ΨKxExxSP). The highly conserved motif regulates phosphorylation-dependent sumoylation of multiple substrates, such as heat-shock factors (HSFs), GATA-1, and myocyte enhancer factor 2. In fact, the majority of the PDSM-containing proteins are transcriptional regulators. Within the HSF family, PDSM is conserved between two functionally distinct members, HSF1 and HSF4b, whose transactivation capacities are repressed through the phosphorylation-dependent sumoylation. As the first recurrent sumoylation determinant beyond the consensus tetrapeptide, the PDSM provides a valuable tool in predicting new SUMO substrates.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0503698102