Temporally Restricted Substrate Interactions Direct Fate and Specification of Neural Precursors Derived from Embryonic Stem Cells

It was, until now, not entirely clear how the nervous system attains its cellular phenotypic diversity and wired complexity during development. Here we describe how environmental interactions alone can modify the development of neurogenic precursor cells. Upon evaluating distinct growth-permissive s...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-07, Vol.103 (29), p.11063-11068
Main Authors: Goetz, A. Katrin, Scheffler, Bjorn, Chen, Huan-Xin, Wang, Shanshan, Suslov, Oleg, Xiang, Hui, Brüstle, Oliver, Roper, Steve N., Steindler, Dennis A.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cell culture
Cell Differentiation
Cell Lineage
Cells, Cultured
Cellular differentiation
Cultured cells
Electrophysiology
Embryonic stem cells
Genotype & phenotype
Mice
Nervous system
Neural stem cells
Neuroglia
Neurons
Neurons - cytology
Neurons - metabolism
Neuroscience
Neurosciences
Patch-Clamp Techniques
Phenotypes
Pluripotent stem cells
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Substrate Specificity
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Summary:It was, until now, not entirely clear how the nervous system attains its cellular phenotypic diversity and wired complexity during development. Here we describe how environmental interactions alone can modify the development of neurogenic precursor cells. Upon evaluating distinct growth-permissive substrates in an embryonic stem cell-neurogenesis assay, we found that laminin, fibronectin, and gelatin instruct neural fate and alter the functional specification of neurons when applied at distinct stages of development. Changes in phenotypic, electrophysiological, and molecular characteristics could resemble cellular events and interactions in the early embryonic brain and may explain why these extracellular matrix components transiently demarcate certain developing brain structures.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0510926103