Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination

Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demon...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-09, Vol.103 (37), p.13624-13628
Main Authors: Shinohara, Takashi, Kato, Megumi, Takehashi, Masanori, Lee, Jiyoung, Chuma, Shinichiro, Nakatsuji, Norio, Kanatsu-Shinohara, Mito, Hirabayashi, Masumi
Format: Article
Language:English
Subjects:
Animals
Artificial insemination
Biological Sciences
Cryopreservation
Fertility
Genomic Imprinting
Germ cells
Insemination, Artificial
Male
Mice
Rats
Reproductive technologies
Rodents
Sertoli cells
Spermatogenesis
Spermatogonia - transplantation
Spermatozoa
Stem cell transplantation
Stem cells
Testes
Testis - cytology
Transplantation
Transplantation, Heterologous
Transplants & implants
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Summary:Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0604205103