Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination

Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demon...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-09, Vol.103 (37), p.13624-13628
Main Authors: Shinohara, Takashi, Kato, Megumi, Takehashi, Masanori, Lee, Jiyoung, Chuma, Shinichiro, Nakatsuji, Norio, Kanatsu-Shinohara, Mito, Hirabayashi, Masumi
Format: Article
Language:English
Subjects:
Animals
Artificial insemination
Biological Sciences
Cryopreservation
Fertility
Genomic Imprinting
Germ cells
Insemination, Artificial
Male
Mice
Rats
Reproductive technologies
Rodents
Sertoli cells
Spermatogenesis
Spermatogonia - transplantation
Spermatozoa
Stem cell transplantation
Stem cells
Testes
Testis - cytology
Transplantation
Transplantation, Heterologous
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663
cites cdi_FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663
container_end_page 13628
container_issue 37
container_start_page 13624
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 103
creator Shinohara, Takashi
Kato, Megumi
Takehashi, Masanori
Lee, Jiyoung
Chuma, Shinichiro
Nakatsuji, Norio
Kanatsu-Shinohara, Mito
Hirabayashi, Masumi
description Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.
doi_str_mv 10.1073/pnas.0604205103
format article
fullrecord <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_103_37_13624_fulltext</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30050305</jstor_id><sourcerecordid>30050305</sourcerecordid><originalsourceid>FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxSMEokvhzAkUcUDikHYcf-aChCo-KhWBoJwtx5kUrxI7tZ2K_vck7KoLXHoaa_ybp5n3iuI5gRMCkp5O3qQTEMBq4ATog2JDoCGVYA08LDYAtawUq9lR8SSlLQA0XMHj4oiIhvEG6k1hv5mcyq8xdLPFrmxvy3OfMaYJrcNUfp8wjiaHq-CdGcrLaHyaBuOzyS740vnys7NYGt-t7xuXY1g7MTifcHT-D_a0eNSbIeGzfT0ufnx4f3n2qbr48vH87N1FZTmFXBFsDWdgZCsU7zlib8H2ioPhgjMFjaGd6CxTnVSSK9lK7NsaWoVGMisEPS7e7nSnuR2xs-hzNIOeohtNvNXBOP3vj3c_9VW40YQLVtdyEXi9F4jhesaU9eiSxWE5GMOctFCKy8W2e0HSUMoYX1d69R-4DXP0iwu6BsJAcbWqne6gxbeUIvZ3KxPQa8x6jVkfYl4mXv596YHf57oAb_bAOnmQo5pKTaiome7nYcj4Ky9seQ-7IC92yDblEO8YCsCBAqe_ASMtx8c</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201408582</pqid></control><display><type>article</type><title>Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>PubMed Central</source><creator>Shinohara, Takashi ; Kato, Megumi ; Takehashi, Masanori ; Lee, Jiyoung ; Chuma, Shinichiro ; Nakatsuji, Norio ; Kanatsu-Shinohara, Mito ; Hirabayashi, Masumi</creator><creatorcontrib>Shinohara, Takashi ; Kato, Megumi ; Takehashi, Masanori ; Lee, Jiyoung ; Chuma, Shinichiro ; Nakatsuji, Norio ; Kanatsu-Shinohara, Mito ; Hirabayashi, Masumi</creatorcontrib><description>Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0604205103</identifier><identifier>PMID: 16945902</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Artificial insemination ; Biological Sciences ; Cryopreservation ; Fertility ; Genomic Imprinting ; Germ cells ; Insemination, Artificial ; Male ; Mice ; Rats ; Reproductive technologies ; Rodents ; Sertoli cells ; Spermatogenesis ; Spermatogonia - transplantation ; Spermatozoa ; Stem cell transplantation ; Stem cells ; Testes ; Testis - cytology ; Transplantation ; Transplantation, Heterologous ; Transplants &amp; implants</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-09, Vol.103 (37), p.13624-13628</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 12, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663</citedby><cites>FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/37.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30050305$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30050305$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776,58221,58454</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16945902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinohara, Takashi</creatorcontrib><creatorcontrib>Kato, Megumi</creatorcontrib><creatorcontrib>Takehashi, Masanori</creatorcontrib><creatorcontrib>Lee, Jiyoung</creatorcontrib><creatorcontrib>Chuma, Shinichiro</creatorcontrib><creatorcontrib>Nakatsuji, Norio</creatorcontrib><creatorcontrib>Kanatsu-Shinohara, Mito</creatorcontrib><creatorcontrib>Hirabayashi, Masumi</creatorcontrib><title>Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.</description><subject>Animals</subject><subject>Artificial insemination</subject><subject>Biological Sciences</subject><subject>Cryopreservation</subject><subject>Fertility</subject><subject>Genomic Imprinting</subject><subject>Germ cells</subject><subject>Insemination, Artificial</subject><subject>Male</subject><subject>Mice</subject><subject>Rats</subject><subject>Reproductive technologies</subject><subject>Rodents</subject><subject>Sertoli cells</subject><subject>Spermatogenesis</subject><subject>Spermatogonia - transplantation</subject><subject>Spermatozoa</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Testes</subject><subject>Testis - cytology</subject><subject>Transplantation</subject><subject>Transplantation, Heterologous</subject><subject>Transplants &amp; implants</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc1v1DAQxSMEokvhzAkUcUDikHYcf-aChCo-KhWBoJwtx5kUrxI7tZ2K_vck7KoLXHoaa_ybp5n3iuI5gRMCkp5O3qQTEMBq4ATog2JDoCGVYA08LDYAtawUq9lR8SSlLQA0XMHj4oiIhvEG6k1hv5mcyq8xdLPFrmxvy3OfMaYJrcNUfp8wjiaHq-CdGcrLaHyaBuOzyS740vnys7NYGt-t7xuXY1g7MTifcHT-D_a0eNSbIeGzfT0ufnx4f3n2qbr48vH87N1FZTmFXBFsDWdgZCsU7zlib8H2ioPhgjMFjaGd6CxTnVSSK9lK7NsaWoVGMisEPS7e7nSnuR2xs-hzNIOeohtNvNXBOP3vj3c_9VW40YQLVtdyEXi9F4jhesaU9eiSxWE5GMOctFCKy8W2e0HSUMoYX1d69R-4DXP0iwu6BsJAcbWqne6gxbeUIvZ3KxPQa8x6jVkfYl4mXv596YHf57oAb_bAOnmQo5pKTaiome7nYcj4Ky9seQ-7IC92yDblEO8YCsCBAqe_ASMtx8c</recordid><startdate>20060912</startdate><enddate>20060912</enddate><creator>Shinohara, Takashi</creator><creator>Kato, Megumi</creator><creator>Takehashi, Masanori</creator><creator>Lee, Jiyoung</creator><creator>Chuma, Shinichiro</creator><creator>Nakatsuji, Norio</creator><creator>Kanatsu-Shinohara, Mito</creator><creator>Hirabayashi, Masumi</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060912</creationdate><title>Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination</title><author>Shinohara, Takashi ; Kato, Megumi ; Takehashi, Masanori ; Lee, Jiyoung ; Chuma, Shinichiro ; Nakatsuji, Norio ; Kanatsu-Shinohara, Mito ; Hirabayashi, Masumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Artificial insemination</topic><topic>Biological Sciences</topic><topic>Cryopreservation</topic><topic>Fertility</topic><topic>Genomic Imprinting</topic><topic>Germ cells</topic><topic>Insemination, Artificial</topic><topic>Male</topic><topic>Mice</topic><topic>Rats</topic><topic>Reproductive technologies</topic><topic>Rodents</topic><topic>Sertoli cells</topic><topic>Spermatogenesis</topic><topic>Spermatogonia - transplantation</topic><topic>Spermatozoa</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Testes</topic><topic>Testis - cytology</topic><topic>Transplantation</topic><topic>Transplantation, Heterologous</topic><topic>Transplants &amp; implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinohara, Takashi</creatorcontrib><creatorcontrib>Kato, Megumi</creatorcontrib><creatorcontrib>Takehashi, Masanori</creatorcontrib><creatorcontrib>Lee, Jiyoung</creatorcontrib><creatorcontrib>Chuma, Shinichiro</creatorcontrib><creatorcontrib>Nakatsuji, Norio</creatorcontrib><creatorcontrib>Kanatsu-Shinohara, Mito</creatorcontrib><creatorcontrib>Hirabayashi, Masumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinohara, Takashi</au><au>Kato, Megumi</au><au>Takehashi, Masanori</au><au>Lee, Jiyoung</au><au>Chuma, Shinichiro</au><au>Nakatsuji, Norio</au><au>Kanatsu-Shinohara, Mito</au><au>Hirabayashi, Masumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2006-09-12</date><risdate>2006</risdate><volume>103</volume><issue>37</issue><spage>13624</spage><epage>13628</epage><pages>13624-13628</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16945902</pmid><doi>10.1073/pnas.0604205103</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 2006-09, Vol.103 (37), p.13624-13628
issn 0027-8424
1091-6490
language eng
recordid cdi_pnas_primary_103_37_13624_fulltext
source JSTOR Archival Journals and Primary Sources Collection; PubMed Central
subjects Animals
Artificial insemination
Biological Sciences
Cryopreservation
Fertility
Genomic Imprinting
Germ cells
Insemination, Artificial
Male
Mice
Rats
Reproductive technologies
Rodents
Sertoli cells
Spermatogenesis
Spermatogonia - transplantation
Spermatozoa
Stem cell transplantation
Stem cells
Testes
Testis - cytology
Transplantation
Transplantation, Heterologous
Transplants & implants
title Rats Produced by Interspecies Spermatogonial Transplantation in Mice and in vitro Microinsemination
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T02%3A26%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rats%20Produced%20by%20Interspecies%20Spermatogonial%20Transplantation%20in%20Mice%20and%20in%20vitro%20Microinsemination&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Shinohara,%20Takashi&rft.date=2006-09-12&rft.volume=103&rft.issue=37&rft.spage=13624&rft.epage=13628&rft.pages=13624-13628&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0604205103&rft_dat=%3Cjstor_pnas_%3E30050305%3C/jstor_pnas_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c530t-1eba540a7b685f5eefc0cf850a5654809a3d6dc48d787587b7efb20b8ea74c663%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=201408582&rft_id=info:pmid/16945902&rft_jstor_id=30050305&rfr_iscdi=true