Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation

Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-09, Vol.103 (39), p.14417-14422
Main Authors: Kobayashi, M, Michaut, L, Ino, A, Honjo, K, Nakajima, T, Maruyama, Y, Mochizuki, H, Ando, M, Ghangrekar, I, Takahashi, K
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Brain
Brain - cytology
Brain - drug effects
Complementary DNA
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Female
gene expression
gene expression regulation
Gene Expression Regulation, Developmental
Genes
Genes, Insect - genetics
Genetic screening
Genetics
hydroxyurea
Hydroxyurea - pharmacology
In situ hybridization
Insects
messenger RNA
Microarray Analysis - methods
microarray technology
Mushroom bodies
Mushroom Bodies - abnormalities
Mushroom Bodies - cytology
Mushroom Bodies - drug effects
Neuroblasts
Neurons
RNA
RNA Interference
RNA, Messenger - genetics
transgenic insects
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Summary:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation by hydroxyurea. We selected 100 genes based on microarray data and examined their expression patterns in the brain by in situ hybridization. Seventy genes were found to be expressed in the posterodorsal cortex, which harbors the MB cell bodies. These genes encode proteins of diverse functions, including transcription, signaling, cell adhesion, channels, and transporters. Moreover, we have examined developmental functions of 40 of the microarray-identified genes by transgenic RNA interference; 8 genes were found to cause mild-to-strong MB defects when suppressed with a MB-Gal4 driver. These results provide important information not only on the repertoire of genes that control MB development but also on the repertoire of neural factors that may have important physiological functions in MB plasticity.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0606571103