Arp2/3-independent assembly of actin by Vibrio type III effector VopL

Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type I...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-10, Vol.104 (43), p.17117-17122
Main Authors: Liverman, Amy D.B, Cheng, Hui-Chun, Trosky, Jennifer E, Leung, Daisy W, Yarbrough, Melanie L, Burdette, Dara L, Rosen, Michael K, Orth, Kim
Format: Article
Language:English
Subjects:
3T3 cells
Actin-Related Protein 2-3 Complex - metabolism
Actins
Actins - metabolism
Amino Acid Sequence
Animals
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Bacterial Proteins - secretion
Biological Sciences
Cytoskeleton
Eukaryotes
Genes, Dominant
Genomics
HeLa Cells
Humans
Infections
Mice
Microfilaments
Models, Biological
Molecular Sequence Data
Monomers
Mutation - genetics
NIH 3T3 Cells
Nucleation
Pathogens
Peptides - metabolism
Proline-Rich Protein Domains
Protein Structure, Tertiary
Proteins
Recombinant Proteins - metabolism
rhoA GTP-Binding Protein - metabolism
Stress fibers
Stress Fibers - metabolism
Transfection
Vibrio
Vibrio Infections
Vibrio parahaemolyticus
Vibrio parahaemolyticus - metabolism
Vibrio parahaemolyticus - pathogenicity
Virulence Factors - metabolism
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Summary:Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0703196104