TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells

During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16⁻CD9⁺KIR⁺ distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16⁺CD9⁻ peripheral blood NK cells in medium containing TGFβ1 resulted in...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-02, Vol.104 (9), p.3378-3383
Main Authors: Keskin, Derin B, Allan, David S.J, Rybalov, Basya, Andzelm, Milena M, Stern, Joel N.H, Kopcow, Hernan D, Koopman, Louise A, Strominger, Jack L
Format: Article
Language:English
Subjects:
Biological Sciences
Blood
Cultured cells
Cytokines
Cytometry
Endometrium
Natural killer cells
Phenotypes
Progenitor cells
Stem cells
Stromal cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093
cites cdi_FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093
container_end_page 3383
container_issue 9
container_start_page 3378
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 104
creator Keskin, Derin B
Allan, David S.J
Rybalov, Basya
Andzelm, Milena M
Stern, Joel N.H
Kopcow, Hernan D
Koopman, Louise A
Strominger, Jack L
description During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16⁻CD9⁺KIR⁺ distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16⁺CD9⁻ peripheral blood NK cells in medium containing TGFβ1 resulted in a transition to CD16⁻CD9⁺ NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and cultured in vitro, were found to produce TGFβ1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFβ1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. In addition, Lin⁻CD34⁺CD45⁺ hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.
doi_str_mv 10.1073/pnas.0611098104
format article
fullrecord <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_104_9_3378_fulltext</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>25426658</jstor_id><sourcerecordid>25426658</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093</originalsourceid><addsrcrecordid>eNp9kb9uFDEQxi0EIkegpgLcUW0yXv_bbZDQQUJEFAqS2vJ67ZyjvfXK9gWiVLwSJbwDD8GTxKe75ERDNaP5fvON7A-hlwQOCEh6OI06HYAgBNqGAHuEZqUjlWAtPEYzgFpWDavZHnqW0hUAtLyBp2iPSCpAcDZDt-fHR39-4imGZcg2YRPGaxuTDyMODs8_EPH3xy882einhY16wN0QQo_PPmNjhyFhP-awxX7vpt98XuDkl37Q0WdffAvVW-P7VbG4x56jJ04Pyb7Y1n10cfTxfP6pOv1yfDJ_f1oZRptcUWh0V3PGZSPa2nIwTtsOrOypFJ0D0gvojTCGMie464yUIAUvnXBGQkv30buN77TqlrY3dszlJWqKfqnjjQraq3-V0S_UZbhWpAHOW1IMDjcGJoaUonUPuwTUOge1zkHtcigbb7cn18KOZqpVlMpGudUwZPs9F_L1f8kCvNoAVymH-ECUD6mF4Gv9zUZ3Oih9GX1SF19rIBRA8poySe8AC7iouA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells</title><source>PubMed Central(OpenAccess)</source><source>JSTOR Archival Journals and Primary Sources Collection</source><creator>Keskin, Derin B ; Allan, David S.J ; Rybalov, Basya ; Andzelm, Milena M ; Stern, Joel N.H ; Kopcow, Hernan D ; Koopman, Louise A ; Strominger, Jack L</creator><creatorcontrib>Keskin, Derin B ; Allan, David S.J ; Rybalov, Basya ; Andzelm, Milena M ; Stern, Joel N.H ; Kopcow, Hernan D ; Koopman, Louise A ; Strominger, Jack L</creatorcontrib><description>During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16⁻CD9⁺KIR⁺ distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16⁺CD9⁻ peripheral blood NK cells in medium containing TGFβ1 resulted in a transition to CD16⁻CD9⁺ NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and cultured in vitro, were found to produce TGFβ1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFβ1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. In addition, Lin⁻CD34⁺CD45⁺ hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0611098104</identifier><identifier>PMID: 17360654</identifier><language>eng</language><publisher>National Academy of Sciences</publisher><subject>Biological Sciences ; Blood ; Cultured cells ; Cytokines ; Cytometry ; Endometrium ; Natural killer cells ; Phenotypes ; Progenitor cells ; Stem cells ; Stromal cells</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2007-02, Vol.104 (9), p.3378-3383</ispartof><rights>Copyright 2007 The National Academy of Sciences of the United States of America</rights><rights>2007 by The National Academy of Sciences of the USA 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093</citedby><cites>FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/104/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25426658$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25426658$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids></links><search><creatorcontrib>Keskin, Derin B</creatorcontrib><creatorcontrib>Allan, David S.J</creatorcontrib><creatorcontrib>Rybalov, Basya</creatorcontrib><creatorcontrib>Andzelm, Milena M</creatorcontrib><creatorcontrib>Stern, Joel N.H</creatorcontrib><creatorcontrib>Kopcow, Hernan D</creatorcontrib><creatorcontrib>Koopman, Louise A</creatorcontrib><creatorcontrib>Strominger, Jack L</creatorcontrib><title>TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><description>During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16⁻CD9⁺KIR⁺ distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16⁺CD9⁻ peripheral blood NK cells in medium containing TGFβ1 resulted in a transition to CD16⁻CD9⁺ NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and cultured in vitro, were found to produce TGFβ1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFβ1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. In addition, Lin⁻CD34⁺CD45⁺ hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.</description><subject>Biological Sciences</subject><subject>Blood</subject><subject>Cultured cells</subject><subject>Cytokines</subject><subject>Cytometry</subject><subject>Endometrium</subject><subject>Natural killer cells</subject><subject>Phenotypes</subject><subject>Progenitor cells</subject><subject>Stem cells</subject><subject>Stromal cells</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kb9uFDEQxi0EIkegpgLcUW0yXv_bbZDQQUJEFAqS2vJ67ZyjvfXK9gWiVLwSJbwDD8GTxKe75ERDNaP5fvON7A-hlwQOCEh6OI06HYAgBNqGAHuEZqUjlWAtPEYzgFpWDavZHnqW0hUAtLyBp2iPSCpAcDZDt-fHR39-4imGZcg2YRPGaxuTDyMODs8_EPH3xy882einhY16wN0QQo_PPmNjhyFhP-awxX7vpt98XuDkl37Q0WdffAvVW-P7VbG4x56jJ04Pyb7Y1n10cfTxfP6pOv1yfDJ_f1oZRptcUWh0V3PGZSPa2nIwTtsOrOypFJ0D0gvojTCGMie464yUIAUvnXBGQkv30buN77TqlrY3dszlJWqKfqnjjQraq3-V0S_UZbhWpAHOW1IMDjcGJoaUonUPuwTUOge1zkHtcigbb7cn18KOZqpVlMpGudUwZPs9F_L1f8kCvNoAVymH-ECUD6mF4Gv9zUZ3Oih9GX1SF19rIBRA8poySe8AC7iouA</recordid><startdate>20070227</startdate><enddate>20070227</enddate><creator>Keskin, Derin B</creator><creator>Allan, David S.J</creator><creator>Rybalov, Basya</creator><creator>Andzelm, Milena M</creator><creator>Stern, Joel N.H</creator><creator>Kopcow, Hernan D</creator><creator>Koopman, Louise A</creator><creator>Strominger, Jack L</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20070227</creationdate><title>TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells</title><author>Keskin, Derin B ; Allan, David S.J ; Rybalov, Basya ; Andzelm, Milena M ; Stern, Joel N.H ; Kopcow, Hernan D ; Koopman, Louise A ; Strominger, Jack L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological Sciences</topic><topic>Blood</topic><topic>Cultured cells</topic><topic>Cytokines</topic><topic>Cytometry</topic><topic>Endometrium</topic><topic>Natural killer cells</topic><topic>Phenotypes</topic><topic>Progenitor cells</topic><topic>Stem cells</topic><topic>Stromal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keskin, Derin B</creatorcontrib><creatorcontrib>Allan, David S.J</creatorcontrib><creatorcontrib>Rybalov, Basya</creatorcontrib><creatorcontrib>Andzelm, Milena M</creatorcontrib><creatorcontrib>Stern, Joel N.H</creatorcontrib><creatorcontrib>Kopcow, Hernan D</creatorcontrib><creatorcontrib>Koopman, Louise A</creatorcontrib><creatorcontrib>Strominger, Jack L</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keskin, Derin B</au><au>Allan, David S.J</au><au>Rybalov, Basya</au><au>Andzelm, Milena M</au><au>Stern, Joel N.H</au><au>Kopcow, Hernan D</au><au>Koopman, Louise A</au><au>Strominger, Jack L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><date>2007-02-27</date><risdate>2007</risdate><volume>104</volume><issue>9</issue><spage>3378</spage><epage>3383</epage><pages>3378-3383</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16⁻CD9⁺KIR⁺ distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16⁺CD9⁻ peripheral blood NK cells in medium containing TGFβ1 resulted in a transition to CD16⁻CD9⁺ NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and cultured in vitro, were found to produce TGFβ1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFβ1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. In addition, Lin⁻CD34⁺CD45⁺ hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.</abstract><pub>National Academy of Sciences</pub><pmid>17360654</pmid><doi>10.1073/pnas.0611098104</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 2007-02, Vol.104 (9), p.3378-3383
issn 0027-8424
1091-6490
language eng
recordid cdi_pnas_primary_104_9_3378_fulltext
source PubMed Central(OpenAccess); JSTOR Archival Journals and Primary Sources Collection
subjects Biological Sciences
Blood
Cultured cells
Cytokines
Cytometry
Endometrium
Natural killer cells
Phenotypes
Progenitor cells
Stem cells
Stromal cells
title TGFβ promotes conversion of CD16⁺ peripheral blood NK cells into CD16⁻ NK cells with similarities to decidual NK cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T07%3A37%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TGF%CE%B2%20promotes%20conversion%20of%20CD16%E2%81%BA%20peripheral%20blood%20NK%20cells%20into%20CD16%E2%81%BB%20NK%20cells%20with%20similarities%20to%20decidual%20NK%20cells&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Keskin,%20Derin%20B&rft.date=2007-02-27&rft.volume=104&rft.issue=9&rft.spage=3378&rft.epage=3383&rft.pages=3378-3383&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0611098104&rft_dat=%3Cjstor_pnas_%3E25426658%3C/jstor_pnas_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c438t-308ab254578692e50cfaeb0e7d376bf01d60dc6cc34f65fbc7707655fb6fc7093%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/17360654&rft_jstor_id=25426658&rfr_iscdi=true