actin-like MreB cytoskeleton organizes viral DNA replication in bacteria

• Published in: Proceedings of the National Academy of Sciences - PNAS 2009-08, Vol.106 (32), p.13347-13352
• Main Authors: Muñoz-Espín, Daniel, Daniel, Richard, Kawai, Yoshikazu, Carballido-López, Rut, Castilla-Llorente, Virginia, Errington, Jeff, Meijer, Wilfried J.J, Salas, Margarita
• Format: Article
• Language: English
• Subjects: Actin Cytoskeleton - genetics; Actin Cytoskeleton - metabolism; Bacillus Phages - genetics; Bacillus Phages - physiology; Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacillus subtilis - virology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriophage PRD1 - genetics; Bacteriophage PRD1 - physiology; Bacteriophages; Biological Sciences; Cell Membrane - metabolism; Cell Membrane - virology; Cell membranes; Cell walls; Cells; Cytoskeleton; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA replication; DNA Replication - genetics; DNA Replication - physiology; DNA, Viral - biosynthesis; DNA, Viral - genetics; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli - virology; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Genes, Bacterial; Genomes; Genomics; Infections; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membranes; Models, Biological; Mutation; Phages; Proteins; Replication; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Attachment; Virus Replication - genetics; Virus Replication - physiology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0906465106

Little is known about the organization or proteins involved in membrane-associated replication of prokaryotic genomes. Here we show that the actin-like MreB cytoskeleton of the distantly related bacteria Escherichia coli and Bacillus subtilis is required for efficient viral DNA replication. Detailed analyses of B. subtilis phage ϕ29 showed that the MreB cytoskeleton plays a crucial role in organizing phage DNA replication at the membrane. Thus, phage double-stranded DNA and components of the ϕ29 replication machinery localize in peripheral helix-like structures in a cytoskeleton-dependent way. Importantly, we show that MreB interacts directly with the ϕ29 membrane-protein p16.7, responsible for attaching viral DNA at the cell membrane. Altogether, the results reveal another function for the MreB cytoskeleton and describe a mechanism by which viral DNA replication is organized at the bacterial membrane.