Effect of 5-Bromodeoxyuridine on Expression of Cultured Chondrocytes Grown in vitro

Exposure of cultured cartilage cells to 5-bromodeoxyuridine results in a rapid loss of the ability to synthesize the chondroitin 4-sulfate-protein complex characteristic of the extracellular matrix of differentiated cartilage. UDP-glucose dehydrogenase, UDP-N-acetyl hexosamine 4-epimerase and the en...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1969-07, Vol.63 (3), p.864-870
Main Authors: Holthausen, H. Schulte, Chacko, S., Davidson, E. A., Holtzer, H.
Format: Article
Language:English
Subjects:
Biological Sciences: Biochemistry
Bromodeoxyuridine - pharmacology
Cartilage - drug effects
Cartilage - enzymology
Cell Differentiation
Cell membranes
Cellular differentiation
Chondrocytes
Chondroitin - biosynthesis
Culture Techniques
Cultured cells
Daughter cells
Embryonic cells
Enzymes
Neurons
Polysaccharides
Sulfates
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Summary:Exposure of cultured cartilage cells to 5-bromodeoxyuridine results in a rapid loss of the ability to synthesize the chondroitin 4-sulfate-protein complex characteristic of the extracellular matrix of differentiated cartilage. UDP-glucose dehydrogenase, UDP-N-acetyl hexosamine 4-epimerase and the enzyme system responsible for catalyzing 3′-phosphoadenosine-5′-ph osphosulfate synthesis all progressively decline in activity, while several other cellular processes seem unaffected. However, there are marked morphological changes which may be associated with plasma membrane components or the synthesis of the protein-polysaccharide complex.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.63.3.864