Proinsulin: Crystallization and Preliminary X-Ray Diffraction Studies

Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 μ g and volumes of 5-20 μ l. The crystalline preparations all differ from each other morphologically. X-ray photographs of one form, tetra...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1970-08, Vol.66 (4), p.1213-1219
Main Authors: Fullerton, W. Wardle, Potter, Reginald, Low, Barbara W.
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological Sciences: Biochemistry
Cattle
Citrates
Crystallization
Crystals
Hydrogen-Ion Concentration
Insulin
Insulin - isolation & purification
Microchemistry
Quaternary ammonium compounds
Room temperature
Ungulates
X-Ray Diffraction
Zinc
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Summary:Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 μ g and volumes of 5-20 μ l. The crystalline preparations all differ from each other morphologically. X-ray photographs of one form, tetragonal bipyramids grown at pH 3 with added ammonium sulphate solution, established the space group P41212 (or its enantiomorph P43212). The cell dimensions are a = 50.8 ± 0.2 angstrom, c = 148.0 ± 0.4 angstrom. The asymmetric unit in this form is a dimer of proinsulin which is also the dominant species in solution at this pH.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.66.4.1213