DNA Polymerase Activity Associated with RNA Tumor Viruses

C-type RNA viruses, originating in mouse, cat, hamster, and viper, catalyze the synthesis of DNA from its constituent deoxyribonucleoside triphosphates. Both the rate and extent of the reaction were significantly enhanced by brief treatment of the intact virus with ether. A proportion of the newly s...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1970-09, Vol.67 (1), p.143-147
Main Authors: Hatanaka, M., Huebner, R. J., Gilden, R. V.
Format: Article
Language:English
Subjects:
Animals
Antigens
Biological Sciences: Biochemistry
Cats
Cricetinae
DNA
DNA - biosynthesis
DNA Nucleotidyltransferases
Enzyme activity
Ethers
Ethyl Ethers - pharmacology
Leukemia Virus, Murine - enzymology
Mice
Oncogenic viruses
Oncogenic Viruses - drug effects
Oncogenic Viruses - enzymology
Rauscher Virus - drug effects
Rauscher Virus - enzymology
RNA
RNA Viruses - enzymology
Snakes
Vipers
Viral RNA
Virions
Viruses
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Description
Summary:C-type RNA viruses, originating in mouse, cat, hamster, and viper, catalyze the synthesis of DNA from its constituent deoxyribonucleoside triphosphates. Both the rate and extent of the reaction were significantly enhanced by brief treatment of the intact virus with ether. A proportion of the newly synthesized DNA was associated with virions when intact virus was used as template; this was not the case with ether-treated virus. In both cases, DNA extracted from the reaction mixture sedimented slowly, at about 2-4 S.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.67.1.143