Sites of Synthesis and Processing of Ribosomal RNA Precursors within the Nucleolus of Urechis caupo Eggs

Nucleoli from unfertilized Urechis eggs, labeled with tritiated RNA precursors, have been isolated for simultaneous autoradiographic localization and biochemical analysis of labeled RNA. The production of the ribosomal RNA precursor (38S) and its first cleavage occur at the fibrillar core region of...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1970-10, Vol.67 (2), p.968-975
Main Authors: Das, Nirmal K., Micou-Eastwood, Julie, Ramamurthy, Gollu, Alfert, Max
Format: Article
Language:English
Subjects:
Acetates
Actinomycin
Biochemistry
Biological Sciences: Biochemistry
Cell nucleolus
Cells
Eggs
Ribosomal RNA
RNA
RNA precursors
Sea water
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Das, Nirmal K.
Micou-Eastwood, Julie
Ramamurthy, Gollu
Alfert, Max
description Nucleoli from unfertilized Urechis eggs, labeled with tritiated RNA precursors, have been isolated for simultaneous autoradiographic localization and biochemical analysis of labeled RNA. The production of the ribosomal RNA precursor (38S) and its first cleavage occur at the fibrillar core region of the nucleolus. The products, predominantly 30S RNA, are then rapidly transported and stored in the granular cortex of the nucleolus. The formation of the nucleolar cortex, therefore, seems to result from an accumulation of partially processed ribosomal RNA with its associated proteins.
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subjects Acetates
Actinomycin
Biochemistry
Biological Sciences: Biochemistry
Cell nucleolus
Cells
Eggs
Ribosomal RNA
RNA
RNA precursors
Sea water
title Sites of Synthesis and Processing of Ribosomal RNA Precursors within the Nucleolus of Urechis caupo Eggs
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