Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-02, Vol.69 (2), p.417-421
Main Authors: Sheldon, Robert, Jurale, Christine, Kates, Joseph
Format: Article
Language:English
Subjects:
Bacteriophage T4
Biological Sciences: Biochemistry
Fiberglass
HeLa cells
Messenger RNA
Radioactive decay
RNA
Vaccinia
Vaccinia virus
Viruses
Water distillation
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Description
Summary:A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportion of the transcripts synthesized in vivo and in vitro from the vaccinia virus genome contain poly(A) sequences and bind to the poly(U) filters. Similarly, DNA-like RNA from the nucleus and from the cytoplasmic polyribosomes of HeLa cells is rich in species that bind to poly(U) filters. Poly(U) immobilized on cellulose powder is useful to make columns with a high capacity for the binding and purification of poly(A)-containing RNAs.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.2.417