Nucleotide Sequences from Tryptophan Messenger RNA of Escherichia coli: The Sequence Corresponding to the Amino-Terminal Region of the First Polypeptide Specified by the Operon

Mutants with internal deletions that terminate near the operator end of the tryptophan operon of E. coli were used in studies on the nucleotide sequence at the 5′end of the messenger RNA transcribed on this operon. The findings obtained permitted identification of a sequence of 43 nucleotides corres...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1973-08, Vol.70 (8), p.2335-2339
Main Authors: Bronson, Morley J., Squires, Craig, Yanofsky, Charles
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - analysis
Bacterial Proteins - biosynthesis
Bacteriophages
Base Sequence
Biological Sciences: Biochemistry
Cyclohexanecarboxylic Acids
DNA
Escherichia coli - analysis
Genetic Code
Hydrolysis
Messenger RNA
Mutation
Nucleotide sequences
Nucleotides
Nucleotides - analysis
Oligonucleotides
Oligonucleotides - analysis
Operator regions
Operon
Operons
ortho-Aminobenzoates
Ribonucleases
RNA
RNA, Bacterial - analysis
RNA, Messenger - analysis
Start codon
Transaminases - analysis
Transaminases - biosynthesis
Transduction, Genetic
Tryptophan
Vinyl Compounds
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mutants with internal deletions that terminate near the operator end of the tryptophan operon of E. coli were used in studies on the nucleotide sequence at the 5′end of the messenger RNA transcribed on this operon. The findings obtained permitted identification of a sequence of 43 nucleotides corresponding to the amino-terminal 11 amino acids of anthranilate synthetase component I, the polypeptide specified by the operator-proximal structural gene of the operon. It was also shown that the translation initiation codon for this polypeptide is preceded on the messenger by a ``leader'' sequence of unknown function, at least 150 nucleotides in length.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.8.2335