Allograft Tolerance: Presumptive Evidence that Serum Factors from Tolerant Animals that Block Lymphocyte-Mediated Immunity in vitro are Soluble Antigen-Antibody Complexes

Serum factors present in rats rendered operationally tolerant to skin allografts by inoculation of allogeneic bone-marrow cells as newborns inhibit or ``block'' the cytotoxic effect of immune lymphocytes in vitro. These blocking factors were specifically removed from tolerant serum by abso...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1973-09, Vol.70 (9), p.2539-2543
Main Authors: Wright, P. W., Hargreaves, R. E., Bansal, S. C., Bernstein, I. D., Hellstrom, K. E.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigen-Antibody Complex
Antigens
Biological Sciences: Immunology
Cytotoxicity
Cytotoxicity Tests, Immunologic
Fibroblasts
Histocompatibility
Homologous transplantation
Immune Tolerance
Lungs
Lymphocytes
Lymphocytes - immunology
Molecular Weight
Rats
Rats, Inbred BN
Rats, Inbred WF
Skin grafts
Skin Transplantation
Transplantation tolerance
Transplantation, Homologous
Ultrafiltration
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Summary:Serum factors present in rats rendered operationally tolerant to skin allografts by inoculation of allogeneic bone-marrow cells as newborns inhibit or ``block'' the cytotoxic effect of immune lymphocytes in vitro. These blocking factors were specifically removed from tolerant serum by absorption with allogeneic cells and later eluted from the absorbing cells in glycine buffer (pH 3.1). Blocking activity of the eluted material was resolved into fractions of low and higher molecular weight, which may be soluble histocompatibility antigen and specific alloantibody, respectively. Both antigen and antigen-antibody complexes may block in vitro, depending upon the assay used.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.9.2539