Equilibrium Sedimentation of Turnip Yellow Mosaic Virus

Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 × 106dalto...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-10, Vol.71 (10), p.3866-3868
Main Authors: McGee, J. H., Kupke, D. W., Godschalk, W., Beams, J. W.
Format: Article
Language:English
Subjects:
Biological Sciences: Biochemistry
Centrifugation
Chemical equilibrium
Densitometry
Density
Density measurement
Dialysis solutions
Hydrogen-Ion Concentration
Magnetics
Molecular Weight
Mosaic Viruses - analysis
Plant Viruses - analysis
RNA
RNA, Viral - analysis
Salts
Solvents
Tymovirus
Ultracentrifugation
Viral Proteins - analysis
Viruses
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Description
Summary:Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 × 106daltons was calculated for the virus at vanishing concentrations, in essential agreement with the earlier results using the more tedious procedure with a freely coasting rotor. In order to interpret the observed departure from ideal behavior (non-associating conditions), preferential interaction experiments were carried out by magnetic densimetry. These showed that a strong Donnan effect exists at pH 7, which contributed >103times more to the nonideality than did the excluded volume effect. A net negative charge of about 3.5 × 103per particle was estimated at this pH.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.10.3866