Density Gradient Separation of Lymphoid Cells Adhering to Protein-A-Containing Staphylococci

A cell separation technique was designed based on the interaction between cell-surface-bound IgG and protein A of Staphylococcus aureus. The density of lymphoid cells coated with IgG antibodies against one of the surface markers was increased by adherence of staphylococci. Cells with adhering bacter...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-12, Vol.71 (12), p.4831-4835
Main Authors: Ghetie, V., Nilsson, K., Sjoquist, J.
Format: Article
Language:English
Subjects:
Antibodies
Antigen-Antibody Reactions
Antigens, Bacterial
Bacteria
Biological Sciences: Immunology
Burkitt Lymphoma
Cell Line
Cell lines
Cell Membrane - immunology
Cell separation
Cell Separation - methods
Centrifugation
Centrifugation, Density Gradient
Chemical suspensions
Fractions
Immunoglobulin G
Iodine Radioisotopes
Lymphocytes
Lymphocytes - immunology
Lysostaphin
Sodium
Staphylococcus - immunology
Viability
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Summary:A cell separation technique was designed based on the interaction between cell-surface-bound IgG and protein A of Staphylococcus aureus. The density of lymphoid cells coated with IgG antibodies against one of the surface markers was increased by adherence of staphylococci. Cells with adhering bacteria were separated from cells without bacteria by density gradient centrifugation in 11.5% sodium metrizoate. Bacteria were removed from the lymphoid cells by lysostaphin digestion. The purity of separated cells was approximately 95% even when the proportion of a specific cell population was below 10% in the initial mixture. The viability and the ability of cells to multiply in vitro were not significantly impaired by the fractionating procedure. The technique can generally be applied for cell separation, provided antibodies of the IgG class against specific surface markers are available.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.12.4831