Isolation of Heterogeneous Circular DNA from Induced Lysogens of Bacteriophage Mu-1

Covalently-closed circular DNA molecules are formed after induction of phage Mu cts4 and after infection with phage Mu cts4. The circular molecules obtained after induction have a molecular length range from 36.5 to 156.7 kilobases as measured by electron microscopic techniques. These heterogeneous...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-04, Vol.71 (4), p.1255-1259
Main Authors: Waggoner, Barbara T., Gonzalez, Nelida S., Taylor, Austin L.
Format: Article
Language:English
Subjects:
Bacteriophage mu
Bacteriophages
Biological Sciences: Biochemistry
Centrifugation, Density Gradient
Cesium
Circles
Circular DNA
Coliphages - analysis
DNA
DNA, Bacterial - biosynthesis
DNA, Circular - isolation & purification
DNA, Viral - biosynthesis
DNA, Viral - isolation & purification
Escherichia coli - metabolism
Genetic heterogeneity
Genomes
Genotype
Hot Temperature
Lysogeny
Microscopy, Electron
Mitomycins - pharmacology
Molecular Weight
Molecules
Mutation
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Prophages
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Summary:Covalently-closed circular DNA molecules are formed after induction of phage Mu cts4 and after infection with phage Mu cts4. The circular molecules obtained after induction have a molecular length range from 36.5 to 156.7 kilobases as measured by electron microscopic techniques. These heterogeneous molecules have no consistent correlation to exact multiples of a Mu genome equivalent (37.3 ± 1.2 kilobases). Direct evidence is given that these molecules contain phage Mu DNA that is covalently linked to other DNA sequences.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.4.1255